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《煙草青枯病拮抗真菌的篩選、鑒定與活性產(chǎn)物的的研究》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、產(chǎn)物的穩(wěn)定性。發(fā)現(xiàn)菌株3F03的活性產(chǎn)物的熱、pH和紫外穩(wěn)定性較好，經(jīng)蛋白酶K和胰蛋白酶處理抑制率僅分別下降了17．O％、14．5％；菌株7F06的活性產(chǎn)物抗紫外線和蛋白酶的能力較強，酸性環(huán)境下抑菌活性僅稍有下降，活性產(chǎn)物經(jīng)80℃以上高溫處理后抑菌能力基本喪失。關(guān)鍵詞：煙草青枯?。晦卓拐婢?；活性產(chǎn)物；鑒定：發(fā)酵條件；穩(wěn)定性ScreeningandIdentificationofAntagonisticFungiagainstRalstoniasolanacearumandResearchontheActiveProductsAbstractT
2、obaccowiltdiseasecausedbyRalatoniasolanacearumisabacterialdisease，widelyoccurredintropicalandsubtropicalzone．IthashappenedandledtoseveredamageinthesouthoftheYangtzeRiverBasinofChina,suchasSichuan，Guangdone,,Fujian，HunanandGuizhouprovince．Yieldlossoftobaccocanreachupto80％inm
3、ostserioustobaccoregion．Traditionalmethodsofchemicalcontrolhavesevereimpactsonecologicalenvironmentfitswellashighcosts，therefore，rotationandmicrobialcontrolwereprovedtobepromisingmethods．Microbialcontrolhastheadvantageofpollution-freeandlong-term，whichcallfundamentallysolve
4、thedrugresidueinthesoil，drugresistanceofpathogensandotherproblems．Itnotonlybenefitstheenvironments，butalsoturnsecologicalresourcesintovirtuouscircle．InordertoprovidethetheoreticalbasisofbiologicalcontrolofRalatoniasolanacearum，thisdissertationfocusedparticularlyonstudiesont
5、heactivestrainscreening,identification,optimizationoffermentationconditions，tobaccoseedlingtestinthepresenceoffermentationbroth，andstabilityofproductsofactivestrains，themainresultsaleasfollow．1．Fiflyty-ninefungusstrainswereisolatedfromsoilsamplesintobaccofieldscollectedfrom
6、Qianjiangdistrict，Chongqing．PaperdiSkmethodWasappliedtoscreenantagonistfungi，and2stains，named3F03and7F06，behavedstronginhibitionactivities．ThediameterofinhibitionzoneWas10．Ommand9．5mm,usingtheconcentrationof250“gcrudeextractperpiecepaperdiskonplate．Repeatedplateantagonismte
7、stshowedthediameterofinhibitionzonehadnoobviousdifference．2．Accordingtothemorphological，cultural，cellularfattyacidandrDNAITSsequenceanalysis，activestrains3F03and7F06wereidentifiedasAspergillusfumigatusandNeosartoryaaureola，respectively．3．Thegrowthoftobaccoseedlingstreatedwi
8、thfermentationbrothofstrains3F03and7F06wasingoodcondition．Comparedwitllcontrols，th
