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《細胞因子誘導小鼠骨髓間充質(zhì)干細胞跨越分化肝細胞的研究》由會員上傳分享，免費在線閱讀，更多相關內(nèi)容在工程資料-天天文庫。
1、萬方數(shù)據(jù)·728·生堡壁墅叢壁盤查!!!!笙!!旦笙!!鲞箜!!塑g!墊』望!塑!!鯉墮!z塾!g：Q!!!!竺墊!!!!!!：!!!盟!：!!細胞因子誘導小鼠骨髓間充質(zhì)干細胞跨越分化肝細胞的研究董學君陳燁邵健忠張國榮項黎新●【摘要】目的探索骨髓問充質(zhì)干細胞(MSCs)體外跨越分化為肝細胞的誘導體系。方法從單核細胞中分離間充質(zhì)干細胞，用添加20ng／mlHGF、10ng／mlFGF-4、10ng／mlOSM和10％NBS的IMDM培養(yǎng)液誘導間充質(zhì)干細胞向肝細胞分化。結(jié)果經(jīng)細胞因子誘導的細胞出現(xiàn)肝細胞樣變化，隨誘導時間延長體積逐
2、漸增大、形態(tài)往上皮樣轉(zhuǎn)變，胞漿豐富，出現(xiàn)雙核或多核細胞。RT—PCR和免疫熒光檢測結(jié)果顯示，AFP在誘導初期表達，在誘導后期表達下降；CKl8、ALB和TAT的表達與誘導時間呈線性關系。第20天達到表達高峰；誘導20d后免疫熒光檢測，AFP、CKl8、ALB和TAT均為陽性；誘導20d細胞的PAS糖原合成反應呈陽性，即具備糖原合成和儲存的肝細胞特有功能。結(jié)論HGF、FGF-4和OSM三種細胞因子組合，可誘導小鼠問充質(zhì)干細胞向肝細胞分化，該結(jié)果為間充質(zhì)干細胞跨越分化肝細胞的分子機制探討和臨床應用打下了基礎?！娟P鍵詞】間充質(zhì)干細胞
3、；肝細胞；分化；肝細胞生長因子{成纖維細胞生長因子一4；制瘤素Trans-differentiationofhepatocytesfrommOllSebonemarrowmesenchymaIstemcelIsinducedbycombi-nationofcytokinesDONGXue-jUrl’，cHENYe，sHAoJian—zhong，etal．’Shaoxingpeople’sHos-pital，Shaoxing312000，P．R．China[Abstract]0bjectiveToestablishaneffec
4、tivemethodforinducingmousebonemarrowmesen—chymalstemcells(MSCs)intohepatocytes．MethodsIsolatedMSCswereselectedbyplasticadher—ence，differentiatedinIscove7SModifiedDulbeceo’smediumsupplementedwith10％newbovineser—um(NBS)，20ng／mLhepatocytegrowthfactor(HGF)，10ng／mLfibrob
5、lastgrowthfactor-4(FGF二4)and10ng／mloncostatinm(OSM)for20days’induction．Themediumwaschangedevery4days．ResultsWhenMSCswereculturedwithHGF，F(xiàn)GF-4andOSM，euboidalmorphology，whichwasacharacteristicofhepatocytes，wasobserved，andthedifferentiatedceilsalsoexpressedmarkergeness
6、pecifictolivercellsinatime-dependentmanner．a(chǎn)-fetoprotein(AFP)wasexpressedonday10。andCKl8，ALBandTATweredetectedonday20。whichwasinconsistentwiththeimmunofluoresenceresults．Differentiatedcellsfurtherdemonstratedthesecellsalsoacquiredfunctionalcharacteristicsofhepatocyt
7、esofstoringglycogen．ConclusionMouseMSCscandifferentiateintohepatocyteswhenin-ducedbyHGF，F(xiàn)GF-4andOSM．[Keywords]Mesenchymalstemcell；HepatoeytetDifferentiation；HGF；FGF-4；0SM已有研究顯示，骨髓單個核細胞可跨越分化為多種不同胚層來源的細胞，如神經(jīng)細胞、心肌細胞、骨骼肌細胞、上皮細胞和胰島細胞等[1嵋1；該研究小組曾利用肝細胞生長因子(hepatocytegrowth
8、fac—tor，HGF)、成纖維細胞生長因子一4(fibroblastgrowthfactor一4，F(xiàn)GF-4)和制瘤素(OncostatinM，OSM)的組合體系，成功地誘導骨髓單個核細胞跨項金項目；紹興市科技計劃項目(2005141)作者單位：312000紹興市人民醫(yī)院醫(yī)
