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《針刺對腦缺血再灌注損傷大鼠外周血清sod、mda、mirna-126與vegf表達(dá)的影響》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、萬方數(shù)據(jù)北京協(xié)和醫(yī)學(xué)院中國醫(yī)學(xué)科學(xué)院碩士研究生學(xué)位論文EffectsofAcupunctureonPeripheralSerumExpressionofSOD，MDA，miRNA一126andVEGFinRatswithCerebralIschemiaReperfusionInjuryObjectiveAbstractThisstudywasbasedonthepreviousresearch，thecerebralischemiareperfusioninjury(CIRI)modelratsweremade
2、bysuture-occludedmethod．Toexploretheeffectsofacupunctureat“Baihui”(GV20)and“Zusanli”(ST36)ontheperipheralserumexpressionofSODandMDArelatedtooxidativestress，andontheexpressionofmiRNA一126andVEGFrelatedtovasculargenesisinratswithCIRI．MethodsHealthymaleSprague--
3、Dawleyratswererandomizedintoasham--operatedgroup(S)，amodelgroup(M)，anacupuncturegroup(A)，andanedaravonegroup(ED)usingarandomdigitstable．Eachgroupwasfurtherrandomlydividedintold，3d，5d，and7dfoursubgroupsbasedonthereperfusiontimesaccordingtoarandomdigitstable．I
4、nthegroup(M)andthegroup(A)，CIRlwasinducedusingthethreadocclusionmethod．ElectroacupuncturestimulationwasapplieddailytoGV20andleftST36for20min，attheindicatedtimepointsaftersuccessfuloperations．Edaravonewasgivenattheindicatedtimepointsaftersuccessfuloperationso
5、nceaday．TTCstainingwasusedfortestingmodel．SerumwassampledfordetectingVEGFproteinviaenzyme-linkedimmunosorbentassay，serumSODactivitywasexaminedusingXanthineoxidasemethod，serumMDAcontentwasevaluatedbyTBAmethod，andserummiRNA一126wasexaminedusingquantitativePCR．R
6、esults1．TTCstainingshowedthattherewasnon—infarctionchangeinthegroup(S)，whichbraintissuepresentedinredcolour，whileinthegroup(M)thecolourinthe6萬方數(shù)據(jù)北京協(xié)和醫(yī)學(xué)院中國醫(yī)學(xué)科學(xué)院碩士研究生學(xué)位論文areaofinfarctioncorewasinwhitecolourcomparedwiththecommonredbraintissue．2．TheserumlevelofS
7、ODintheCIRIratsdecreasedsignificantly，andtheminimumexpressionofSODinboththegroup(M)andthegroup(A)occurredat3dExceptthetimepointofldingroup(A)，theexpressionofSODinthegroup(A)andthegroup(ED)washigherthaninthegroup(M)(P<0．05)，andthelevelofSODinthegroup(ED)wassi
8、gnificantlyhigherthaninthegroup(A)(P<0．05)．3．MDAexpressioninserumfromthecerebralischemiagroupwashigherthanthatintheingroup(S)．Comparedwiththegroup(M)，thelevelofMDAintheserumofthegroup(A)andthegr
