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《宮頸癌及其前期病變hpv感染與基因型分布及hpv16癌基因轉(zhuǎn)錄水平沉默研究》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、浙江大學(xué)博士學(xué)位論文PARTIEstabUshmentofaHPVgenotypedetectionmethodobjective：TbestablishaHPVgeno勺憎edeteCtionmetllodMethod：BaSedonmegeIlitalHPVsequeIlc懿，wechosenlesui切bleenzyIIlesto鶴切blishPCR-restriction觸舯∞tleIlgtllpolyIIlo珥“sm(RFLP)嬲sayHC11w硒uSedt0testsensitiv時(shí)ofPCR一Ⅺ7LP，andDN
2、Asequellcil喀wasllsedtotesttlleaccuracy．ResInt：EstablishaHPVgcnotypedetectionmetllod(PCR—I江LP)．EstablishcoIll]monHPVg即otypeselec仃0phoresis伊印lls．TheacC0rd鋤CeofmePcR—RFLP鋤dHC11w舔92．4％inHPVdetection．111egenot)『pesPCIORFLPideIltifiedw弱謝fiedbyDNAsequ∞c噸．Conclusion：0吡PCR-R
3、FLP鶴sayisaIlaCc眥Ite鋤dcoSt-e毹ctiveme吐10d，c鋤dis曲guishmostofmeg“talHPVg朗otypes．111em礎(chǔ)odissui劬leforHPVgenotypedete砸on．Keywords：H啪aIlpapillomavin璐(HPⅥ；PCR；res仃i砸on嘶entl∞gthpolymo叩m鋤(I譚LP)；HybridC印ture(HCII)；Scqu肌血gⅡI．3浙江大學(xué)博士學(xué)位論文n蝴IIDistributioⅡofHPVgenotypesinthepatients
4、withcenricalcanceranditsprecursorsobjectiVe：1’0mVestigatemedistributionofHPVgellotyp髓inthepatieIltswimcer“calc鋤cer鋤ditsprecu-rsors丘lomZh句i鋤gProvince．Method：CervicalCallcer(N=181)，ccrvicali11仃a印ithelialncopl嬲ia(CIN)II_III(N=345)，舡ldCINI(N=105)脅mZhqiangProvinceweredet
5、e∞edforHPVgeIlotypesbyt11eimpr0VedPCR-RFLP．Age-matcheds鋤plesof217women、Ⅳitlloutcer“calr啪pl嬲iaweredetected船Comr01．ResIIIt：TheprevaleIlceofHPVw嬲95．O％inCC，88．4％iIlCINIHII，鋤d73．3％iIlC礬I，wllileomy41．9％illcon仃01．Hi曲一risk／low—riskHPVratioshowedasi嘶6cant咖dofillcre觴ewimiIlcr
6、e邪cd蓼adeofCIN鋤d‰sf0咖ationt0CarC．moma．Atotalof24HPVgenotyp髂weredetectedinCCanditspreclIrs0璐．oftll0∞，HPV16，18，arld58wercmem萄orHPVg鋤otyp鶴inCC，Ⅵ恤leHPVl6，58，33，and18i11CIN．Higll-risk／low珂skHPVratioshowedasi班ficant咖dofiI婀e弱e、)l，immcre弱ed莎ad"x浙江大學(xué)博士學(xué)位論文PARTIⅡThemethylation
7、OfHPV16LCRinasymptomadcinfbction，cervicaIcanceranditsprecursorsObjec咖e：T0obtaindetailedmemylationinf0加ationonCmcialre百onsofHPVl6LCR鋤dclad匆mesi鰣fic鋤ceinClillicalceⅣicallesioIlsMethod：70cliIlicalsampl鷦，indudiIlg26c勰esofceⅣicalcarcilloma，l3ofCINIII，17ofCINI-II，鋤d14of刪t
8、omaticHPV16洫f礎(chǔ)。鄴，we牦dete積dontllememylationstat吣ofHPV16p舢oter鋤deIlll觚cercorebybiSulf
9、temodi6cationa芏ldpFosequen(婦g．Result：FormegenefalmeⅡly
