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1、第28卷第11期生態(tài)學(xué)報(bào)Vol.28,No.112008年11月ACTAECOLOGICASINICANov.,2008基于ISSR標(biāo)記的扁玉螺(Neveritadidyma)自然居群遺傳結(jié)構(gòu)3孫始威,孫振興,葛宜和,閆冬春,黃清榮(魯東大學(xué)生命科學(xué)學(xué)院,煙臺(tái)264025)摘要:利用ISSR分子標(biāo)記技術(shù),對(duì)采自大連(DL)、煙臺(tái)(YT)及青島(QD)近海的扁玉螺3個(gè)自然居群的遺傳結(jié)構(gòu)和遺傳多樣性進(jìn)行了分析。用13個(gè)引物對(duì)90只個(gè)體進(jìn)行了PCR擴(kuò)增,共檢測(cè)到161個(gè)位點(diǎn),3個(gè)居群的多態(tài)位點(diǎn)比例為74.53%～
2、85109%,各居群遺傳多樣性水平的高低依次為YT>QD>DL。扁玉螺在物種水平上的Nei’s基因多樣性指數(shù)和Shannon’s信息指數(shù)分別為0.3395和0.5113,在居群水平上分別為0.2811和0.4189,顯示出扁玉螺有著較高的遺傳多樣性。AMOVA分子變異分析表明,扁玉螺的遺傳變異有27.16%發(fā)生在居群間,72.84%發(fā)生在居群內(nèi),居群內(nèi)的遺傳變異大于居群間的遺傳變異。扁玉螺3個(gè)居群間的遺傳分化系數(shù)(Gst)為0.1720,基因流(Nm)為2.4063,Nei’s遺傳距離平均值為0.1228,
3、表明扁玉螺居群間雖然存在著一定程度的遺傳分化,但仍屬于種內(nèi)正常分化的范疇。上述結(jié)果為保護(hù)和利用扁玉螺資源提供了科學(xué)依據(jù)。關(guān)鍵詞:扁玉螺;遺傳結(jié)構(gòu);ISSR文章編號(hào):100020933(2008)1125499207中圖分類(lèi)號(hào):Q145文獻(xiàn)標(biāo)識(shí)碼:AGeneticstructureofnaturalpopulationofNeveritadidymabasedonISSRmarkers3SUNShi2Wei,SUNZhen2Xing,GEYi2He,YANDong2Chun,HUANGQing2RongCol
4、legeofLifeScience,LudongUniversity,Yantai264025,ChinaActaEcologicaSinica,2008,28(11):5499～5505.Abstract:ThegeneticstructureandgeneticdiversityinthreenaturalpopulationsofNeveritadidymawereanalyzedbyusingthemolecularmarkertechniqueofintersimplesequencerepeat
5、(ISSR).ThesamplesofthethreepopulationsweretakenfromtheoffshoresofDalian(DL),Yantai(YT)andQingdao(QD),respectively.Atotalof161locifrom90individualswerepolymerase2chain2reaction(PCR)amplifiedwith13primers.Theproportionofpolymorphiclociinthethreepopulationsra
6、ngedfrom74.53%to85.09%.TheabundanceofgeneticdiversityinthethreepopulationsarrangedinadecentorderwasYT>QD>DL.TheNei′sgenediversityandShannon′sinformationindexofNeveritadidymawas013395and0.5113atspecieslevel,0.2811and0.4189atpopulationlevel,respectively,show
7、ingthatthegeneticdiversityofNeveritadidymawasrelativelyhigh.Ananalysisofthemolecularvariance(AMOVA)demonstratedthattheamong2populationcomponentaccountedfor27.16%ofthetotalvariation,whilethewithin2populationcomponentaccountedfor72.84%.Thewithin2populationge
8、neticvariationwasapparentlylargerthantheamong2population.Thegeneticdifferentiationcoefficient(Gst),geneflow(Nm)andtheaverageNei′sgeneticdistancewere0.1720,2.4063and基金項(xiàng)目:山東省自然科學(xué)基金資助項(xiàng)目(Y200D12)收稿日期:2008204202;修
