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《增殖性糖尿病視網(wǎng)膜病變患者玻璃體腺苷濃度和增殖膜中cd73表達(dá)研究》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、增殖性糖尿病視網(wǎng)膜病變患者玻璃體腺苷濃度和增殖膜中CD73表達(dá)研究郎敏1袁容娣2陳春林1霍妍1鄒歡1葉劍1(400042,重慶，第三軍醫(yī)大學(xué)大坪醫(yī)院野戰(zhàn)外科研究所眼科1)（400037，重慶，第三軍醫(yī)大學(xué)新橋醫(yī)院眼科2）[摘要]目的通過檢測增殖性糖尿病視網(wǎng)膜病變(proliferativediabeticretinopathy，PDR)患者玻璃體腺苷濃度及增殖膜CD73的表達(dá)，探討增殖性糖尿病視網(wǎng)膜病變與玻璃體腺苷濃度及增殖膜CD73表達(dá)之間的關(guān)系。方法收集在本院眼科行玻璃體切除手術(shù)的患者共30例，分為增殖性糖尿病視網(wǎng)膜病變(PDR共16例16
2、眼）組和對照組（孔源性視網(wǎng)膜脫離、黃斑前膜、特發(fā)性黃斑裂孔共14例14眼）。于患者玻璃體切除手術(shù)中收集玻璃體、視網(wǎng)膜增殖膜及黃斑前膜。應(yīng)用高效液相熒光檢測法檢測患者玻璃體腺苷的濃度，Westernblot檢測膜樣本中CD73蛋白的表達(dá)。結(jié)果人眼玻璃體腺苷濃度及增殖膜CD73酶在PDR組表達(dá)高于對照組（62.37±11.47μMvs30.34±6.41μM；0.84±0.08vs0.39±0.05），差異均具有統(tǒng)計(jì)學(xué)意義(t=9.480,P=0.000；t=7.15,P=0.002)。結(jié)論腺苷和CD73在PDR患者的玻璃體及增殖膜中的表達(dá)較非視網(wǎng)
3、膜新生血管性疾病患者明顯升高，提示二者可能參與了增殖性糖尿病視網(wǎng)膜病變新生血管的形成。[關(guān)鍵詞]腺苷；CD73；增殖性糖尿病視網(wǎng)膜病變；視網(wǎng)膜新生血管[中圖法分類號]R774.1[文獻(xiàn)標(biāo)志碼]AExpressionandsignificanceofadenosineandCD73inproliferativediabeticretinopathyLangMin，ChenChunling,Huoyan，ZouHuan,YeJian（DepartmentofOphthalmology，DapingHospital，theThirdMilitaryM
4、edicalUniversity，Chongqing400042，China）;YuanRongdi.DepartmentofOphthalmology，XinqiaoHospital，theThirdMilitaryMedicalUniversity，Chongqing400037，China.[Abstract]ObjectiveToinvestigatethelevelsofadenosineinvitreosandtheexpressionofCD73infibrovascμlarmembraneofpatientswithprolif
5、erativediabetic[基金項(xiàng)目]第三軍醫(yī)大學(xué)校青年創(chuàng)新人才基金(2010XQN37)[通信作者]葉劍，E-mail:yejian1979@163.comretinopathy(PDR).MethodsWeselected30patientswhounderwentvitrectomiesintheOphthalmologyatDapingHospitalanddividedthemintotwogroups.Sixteenpatients(16eyes)wereingroupdiseasesofretinalneovascμlariz
6、ation(PDRgroup).Fourteenpatients(14eyes)wereincontrolGroup,whichwaswithoutneovascμlarizationinretinal.Inthisgroup,rhegmatogenousretinaldetachment,idiopathicmacμlarholeandmacμlarepiretinalmembranewerecontained.ThelevelsofadenosineinvitreousweremeasuredbyHPLC.TheexpressionofCD
7、73inpreretinalmembraneswasmeasuredbywesternblot.ResμltsThelevelsofadenosineingroupPDRwashigherthanthatingroupcontrol(t=9.480,P=0.000).TheexpressionofCD73ingroupPDRwashigherthanthatingroupcontrol(t=7.15,P=0.002).ConclusionTheexpressionofandenosineandCD73mayinvolvedintheprogre
8、ssofneovascμlarizationwithproliferativediabeticretinopathy.[Keywords]Adenos
