母愛剝奪對(duì)大鼠行為學(xué)及癲癇易感性的影響

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1、母愛剝奪對(duì)大鼠行為學(xué)及癲癇易感性的影響摘要目的研究母愛剝奪對(duì)大鼠行為學(xué)及隨后癲癇發(fā)作的影響。方法將新生的SpragueDawle(SD)雄性大鼠隨機(jī)分為母愛剝奪組和對(duì)照組。母愛剝奪組大鼠自出生后第2天至第14天，每天上午9：00．12：00給予母愛剝奪，對(duì)照組不給予任何處理；第21天斷奶分籠，監(jiān)測(cè)體重及每日體重增長(zhǎng)量；第26天開始進(jìn)行糖水偏愛實(shí)驗(yàn)：糖水偏愛實(shí)驗(yàn)結(jié)束后，以上兩組大鼠隨機(jī)分為兩組，其中一組接受腹腔注射匹魯卡品建立癲癇模型(C+P組和M+P組)，另一組給予同等劑量的生理鹽水腹腔注射(C+C組和M+C組)；采用Ne

2、uN免疫熒光染色法計(jì)數(shù)大鼠海馬齒狀回顆粒層細(xì)胞數(shù)。結(jié)果母愛剝奪組大鼠體重及每日體重增長(zhǎng)量顯著低于對(duì)照組(P

3、W海馬齒狀回顆粒層細(xì)胞數(shù)較對(duì)照組(C+C組)顯著增加(PO．05)。結(jié)’論母愛剝奪應(yīng)激可以造成大鼠成年后出現(xiàn)類似抑郁樣行為學(xué)改變，并使癲癇發(fā)作易感性增加。碩士研究生：潘楠楠(神經(jīng)病學(xué))指導(dǎo)教師：鄭乃智關(guān)鍵詞：母愛剝奪；糖水偏愛；抑郁；癲癇；顆粒層TheeffectsonbehavioralandsusceptivitytoepilepsyofratsbymaternaldeprivationAbstractObject

4、iveWrestudiedtheeffectsofmatemaldeprivation(MD)onbehavioralandsusceptivityto．epilepsyfollowedMDofrats．MethodsNewbornSDmaleratsweredividedintomaternaldeprivationgroupandcontrolgroup(C)randomly；RatsinMDgroupexperiencedmaternaldeprivationfor3hourseachdayfromthe2ndtot

5、he14thpostnatalday(PD)；RatsinCgroupdidn’treceiveanytreatment；AtPD21．a(chǎn)llratswereweaned，andweigheddailyandrecordedtheincreaseofbodyweighteveryday；AllratsreceivedsucroseconsumptiontestatPD26；Eachgroupdividedintotwogroupsrandomly，onegroupofratsreceivedpilocarpinetoind

6、uceseizures(M+PandC+P)，whiletheothergroupreceivednormalsalineinsteadofpilocarpine(M+CandC+C)；Itwas‘countedthenumberofneuronsathippocampaldentategymsgranularlayerofratsbyNeuNimmunofluorescencestain．ResultsbodyweightsanditsincreasesofratsinMDgroupweresignificantlowe

7、rthanratsinCgroup(P<0．05)；ItwassignificantlylowersucrosepreferencerateofratsinMDgroupthaninCgroup(P<0．05)；Afterreceivedpilocarpine．thelatencysofseizuresinMDratswassignificantlyshortencomparedtoratsinCgroup：TheseverityofseizuresofratsinMDgroupwashigherthanratsinCgr

8、oupbyRacine’sscale；Thenumberofneuronsinhippocampaldentategyrusgranularlayerlwand4waftermaternaldeprivationofratsinM+Cgroupweresignificantlydecreasedcomp