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1、照組大鼠放回鼠籠,訓練6h后對6-12hRSD組大鼠進行REM睡眠剝奪;第四天在場景A中進行恐懼消退再現(xiàn)檢測20min(無任何刺激呈現(xiàn))。研究表明實驗一和實驗二兩種任務不依賴于海馬,實驗三和實驗四兩種任務是海馬依賴性的。在恐懼條件化、恐懼消退訓練及消退再現(xiàn)檢測階段記錄分析大鼠的僵直行為。結果:實驗一:在恐懼條件化以及恐懼消退訓練階段,0-6hRSD組、6-12hRSD組與各自對照組大鼠的僵直水平組間差異都無顯著性;在恐懼消退再現(xiàn)檢測階段,0-6hRSD組大鼠的僵直水平顯著高于對照組,6-12hRSD組與對照組大鼠的僵直水平組間差異無顯著性。實驗二:在恐懼條件化以及恐懼消
2、退訓練階段,0-6hRSD組、6-12hRSD組與各自對照組大鼠的僵直水平組間差異都無顯著性;在恐懼消退再現(xiàn)檢測階段,0-6hRSD組大鼠的僵直水平顯著高于對照組,6-12hRSD組與對照組大鼠的僵直水平組間差異無顯著性。實驗三:在恐懼條件化、恐懼消退訓練以及恐懼消退再現(xiàn)檢測階段,0-6hRSD組、6-12hRSD組與各自對照組大鼠的僵直水平組間差異都無顯著性。實驗四:在恐懼條件化、恐懼消退訓練以及恐懼消退再現(xiàn)檢測階段,0-6hRSD組、6-12hRSD組與各自對照組大鼠的僵直水平組間差異都無顯著性。結論:恐懼消退訓練后的RSD對于恐懼消退記憶的影響是復雜的,RSD損害
3、海馬非依賴性恐懼消退再現(xiàn),而對海馬依賴性任務沒有影響,并且這種損害依賴于恐懼消退訓練后睡眠剝奪的時段,進一步說明REM睡眠選擇性參與特定任務的記憶過程。關鍵詞:睡眠剝奪,恐懼消退,海馬,場景,僵直,大鼠4EffectsofREMsleepdeprivationonrecalloffearextinctioninratsABSTRACTObjectives:Thepresentstudywastoexaminetheeffectsofpost-extinctionrapideyemovementsleepdeprivation(RSD)onrecalloffearexti
4、nction。Methods:Experiment1:OnDay1,ratsweretransportedtocontextAtohabituatethemtothecontext;OnDay2(fearconditioningphase),RatsreceivedfivepairingsoftoneandfootshockincontextA;Thenextday(extinctiontrainingphase),ratsreceivedtentone-aloneextinctiontrialsincontextA,immediately,0-6hRSDratswer
5、eplacedintheRSDapparatusand6-12hRSDratsandcontrolratswereplacedintheirhomecages,sixhourslater,6-12hRSDratswereplacedintheRSDapparatus;OnDay4(recalltestphase),ratsreceivedtenadditionalextinctiontrialsincontextA。Experiment2:OnDay1,ratsweretransportedtocontextAandcontextBtohabituatethemtoth
6、econtext;OnDay2(fearconditioningphase),ratsreceivedfivepairingsoftoneandfootshockincontextA;Thenextday(extinctiontrainingphase),ratsreceivedtentone-aloneextinctiontrialsincontextB,immediately,0-6hRSDratswereplacedintheRSDapparatusand6-12hRSDratsandcontrolratsweretransportedtotheirhomecag
7、es,sixhourslater,6-12hRSDratswereplacedintheRSDapparatus;OnDay4(recalltestphase),ratsreceivedtenadditionalextinctiontrialsincontextB。Experiment3:OnDay1,ratsweretransportedtocontextAandcontextBtohabituatethemtothecontext;OnDay2(fearconditioningphase),ratsreceivedfivepairin