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1、第三軍醫(yī)大學(xué)博士學(xué)位論文干細胞分化及其對急性肝衰竭大鼠治療的實驗研究姓名：周一鳴申請學(xué)位級別：博士專業(yè)：內(nèi)科學(xué)（傳染病學(xué)）指導(dǎo)教師：胡大榮20050501第三軍醫(yī)大學(xué)博士學(xué)位論文英文縮寫2-AAF2-acetylaminofluoreneABCavidin··biotin--peroxidasecomplexAFPALBBSACcl4CFUFCKCKl9DABp琶PCDAPIDMEMDPpIVEDTAEGFFACSfAHAlpha-fetoproteinAlbuminBoveinserumalbuminTetrachlorideClonyformingunitsfibroblast
2、sCytokineCytokeratin19DiaminobenzidineDiethylpyrocarbonate4’，6--Diamidino一2一phenylindoledihydrochloridehydrateDulbecco'smodifiedEaglelSmediumDipetideldipeptidaseIVEthylenediaminetetraceticacidEpidermalgrowthfactorFtuorescerrce-ac6vatedcellsortingFumarylacetoacetatehydrolaseFCSFetalcalfserum粥FF
3、ibroblastgrowthfactorGAPDHGlyceraldehyde一3一phosphateEGFPdehydrogenaseEnhancedgreenfluorescentprotein2一乙酰氨基芴抗生物素一生物素一過氧化物酶復(fù)合物甲胎蛋白白蛋白牛血清白蛋白四氯化碳成纖維細胞集落形成單位細胞因子綏瑰熱罄自193，3一二氦基苯聯(lián)胺焦磷酸二乙酯4’，6一二醚基一2一苯攀吲哚鹽酸純合飭培養(yǎng)液二肽酶IV乙二胺四乙酸表皮生長困予流式細胞儀延胡羧酰乙酰乙酸水鰓酶胎牛血清纖維緞戇生茨因子甘油醚～3一磷酸脫氫酶增強型綠色熒光蛋自第三軍躐太學(xué)博士學(xué)位論文HGFHPeHSCMSCsNP
4、eSOCOVPBSPCRHPPVDFRT．PCRSCFSRY稻￡8TNF．曉VEGFX-galHepatocytesgrowthfactorHepaticprogenitorcellsHepaticstellatecellsMarrowmesenchymalstemcellsNonparench)analOvaIcells0valcellmarkerPhosphate-bufferedsalinePolymerasechainreactionHepatectomypotyvinylidenedifluofideReversaltranscriptpolymerasechainrea
5、ctionStemcellfactotSex-determiningregionYchromosomeTransforminggrowthfactor-lSTumotnecrosisfactor-ctVascularendothelialgrowthfactor5-Bromo一4-chloro一3一indolyl-$一D—gatactoside脬綱胞生長困予捋手租細麓肝星細胞囂髓聞充質(zhì)干細胞jI于菲實質(zhì)綱藏卵圓細胞卵圓細胞標(biāo)志磷酸緩沖浚聚合酶鏈?zhǔn)椒磻?yīng)jl干部分切除術(shù)聚偏二乙爝二穰酯逆轉(zhuǎn)錄PCR予纓旎蠢予Y染色體性別決定區(qū)轉(zhuǎn)稔生長因子一#腫瘤壞死因子川贏管內(nèi)皮生長隧子5．溪．4一氯一
6、3一甥絳一§一D一半魏糖苷第三軍鬣太譬博士學(xué)位論文DifferentiationofstemcellsandtherapyoftransplantedstemcellsderivedfrommarrowfortheacuteliverdisfunctionofratsAbstractHumanhealthhasbeenthreatenedbadlybyliverdisease．Livertransplantationispresentlythesolecurativetherapyforhepaticfailure．Butitishighlycostlycomplexandlimi
7、tedbytheavailabili玲ofdonorlivers．Asanalternative，hepatocytetransplantationhasbeenconsideredwiththehopeofassistinghepaticregenerationinacutefailure。buttheshortlifeofisolatedhepatocytesandlimits的thetransplantablecellnumbersaswellasthesarn
