同種異體嗅球組織移植對(duì)神經(jīng)病理性疼痛大鼠脊髓中p物質(zhì)表達(dá)的影響

《同種異體嗅球組織移植對(duì)神經(jīng)病理性疼痛大鼠脊髓中p物質(zhì)表達(dá)的影響》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。

1、摘要舢舢Ⅲ㈣舢㈣刪舢Y2427625目的：觀察同種異體嗅球組織移植對(duì)神經(jīng)病理性疼痛大鼠脊髓中P物質(zhì)的mRNA和蛋白水平的變化，探討P物質(zhì)在同種異體嗅球組織移植對(duì)神經(jīng)病理性疼痛中的作用。方法：我們將SD大鼠隨機(jī)分成假手術(shù)組(Sham組)、慢性坐骨神經(jīng)結(jié)扎損傷組(CCI組)和CCI+同種異體嗅球組織移植組(CCI+AOBTT組)，于術(shù)前和術(shù)后1、3、7、14d測(cè)定機(jī)械縮足反射閾值(MWT)和熱縮足反射潛伏期(TWL)，術(shù)后7、14d取大鼠術(shù)側(cè)腰4(L4)、腰5(L5)脊髓節(jié)段，采用逆轉(zhuǎn)錄聚合酶鏈反應(yīng)(RT．PCR)和免疫組織化學(xué)方法檢測(cè)同種異體嗅球組織移植后CC

2、I組脊髓中P物質(zhì)(SubstanceP，SP)的mRNA和蛋白水平的變化。結(jié)果：CCI組術(shù)后7、14天MWT、TwL與Sham組相比明顯降低(p<0．05)，脊髓中P物質(zhì)的mRNA和蛋白水平明顯增多(p

3、ive：ToinvestigatethechangeofallogeneicolfactorybulbtissuetransplantationonthelevelofsubstancePmRNAandproteininspinalcordofratswithneuropathicpain，weexploresubstancePinallogeneicolfactoryensheathingtissuetransplantationofneuropathicpain．Methods：WedividedrandomlySDratsintothreegroups

4、：Shamoperation(Sham)group，chronicconstrictioninjury(cci)group，CCI+allogeneicolfactorybulbtissuetransplantation(CCI+AOBTT)group．Mechanicalwithdrawalthreshold(MWT)andthermalwithdrawallateny(TWL)ofratsweremeasuredbeforeoperationandl、3、7and14daysafteroperation．RT—PCR(ReverseTranscripti

5、on—PolymeraseChainReaction)andimmunohistochemistrywereusedtodetectthechangesubstancePmRNAandproteinlevelinL4andL5ofspinalcordofratsontheoperativesidewere7and14daysafteroperation．Results：Comparedwiththeshamgroup，in3，7and14daysMWTandTWLdecreasedmarkedly(p

6、4dayssubstancePmRNAandproteininspinalcordofratsonoperativesideincreasedsignificantlyafteroperationintheCCIgroup(p<0．05)．ComparedwiththeCCIgroup，in3，7and14daysMWTandTWLincreasedmarkedly(p<0．05)inCCI+AOBTTgroup，whilein7and14dayssubstancePmRNAandproteininspinalcordofratsonoperativesid

7、edecreasedsignificantlyinCCI+AOBTTgroup(p<0．05)．Conclusion：AllogeneicolfactorybulbtissuetransplantationgroupmayreduceneupopathicpainofratswithCCIviainhibitiontheexpressionofsubstancePinspinalcord．．Keywords：substanceP：neuropathicpain；RT-PCR；immunohistochemistry；olfactoryensheathingt

8、issue；Rat目錄目錄第1章引言????????