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《農(nóng)桿菌介導(dǎo)的小麥愈傷轉(zhuǎn)化及小麥懸浮細(xì)胞系的建立》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、爭(zhēng)十；11火#HUAZHONGAGRICULTURALUNIVERSITY碩士學(xué)位論文MASTER，SDEGREEDISSERTATION農(nóng)桿菌介導(dǎo)的小麥愈傷轉(zhuǎn)化及小麥懸浮細(xì)胞系的建立AGROBACTERIUM-MEDIATEDTRANSFORMATIONOFWHEATCALLIANDTHEESTABLISHMENTOFWHEATCELLSUSPENSIONCULTURES研究生：曹樂(lè)慧CANDIDATE:CAOLEHUI導(dǎo)師：李和平教授SUPERVISOR:PROFESSORLIHEPING專業(yè)：細(xì)胞
2、生物學(xué)MAJOR:CELLBIOLOGY研究方向：植物基因工程FIELD:PLANTGENETICENGINEERING中國(guó)武漢WUHAN,CHINA二〇一五年六月JUNE,2015分類號(hào)密級(jí)華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文農(nóng)桿菌介導(dǎo)的小麥愈傷轉(zhuǎn)化及小麥懸浮細(xì)胞系的建立Agrobacterium-mediatedTransformationofWheatCalliandtheEstablishmentofWheatCellSuspensionCultures研究生:曹樂(lè)慧指導(dǎo)教師:李和平教授廖玉才教授專業(yè)：細(xì)胞
3、生物學(xué)研究方向：植物基因工程獲得學(xué)位名稱：理學(xué)碩士獲得學(xué)位時(shí)間：2015年6月華中農(nóng)業(yè)大學(xué)生命科學(xué)技術(shù)學(xué)院二O一五年六月農(nóng)桿菌介導(dǎo)的小麥愈傷轉(zhuǎn)化及小麥懸浮細(xì)胞系的建立目錄摘要................................................................................................................................iABSTRACT..............................
4、.........................................................................................iii縮略語(yǔ)表............................................................................................................................vi1前言.............................
5、.....................................................................................................11.1小麥轉(zhuǎn)基因技術(shù)研究進(jìn)展...................................................................................11.1.1直接轉(zhuǎn)化法............................................
6、............................................11.1.2農(nóng)桿菌轉(zhuǎn)化法....................................................................................21.2農(nóng)桿菌轉(zhuǎn)化分子機(jī)制...........................................................................................31.2.1農(nóng)桿菌對(duì)
7、受體的識(shí)別并誘導(dǎo)毒性區(qū)基因的表達(dá)............................41.2.2T-DNA的加工及轉(zhuǎn)運(yùn)......................................................................51.2.3T-DNA在植物細(xì)胞內(nèi)的轉(zhuǎn)運(yùn)、整合及表達(dá)..................................61.3農(nóng)桿菌介導(dǎo)的小麥遺傳轉(zhuǎn)化影響因素...........................................
8、....................71.3.1農(nóng)桿菌菌株及表達(dá)載體的構(gòu)建........................................................71.3.2毒性區(qū)基因活化誘導(dǎo)物的添加........................................................71.3.3受體基因型及其生理狀態(tài)...................................
