資源描述：
《RNAi和GroEL介導(dǎo)的雙價(jià)轉(zhuǎn)基因番茄對(duì)TYLCV的抗性》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。
1、江蘇農(nóng)業(yè)學(xué)報(bào)(JiangsuJ.ofAgr.Sci.),2014,30(5):1102~11081102http://www.jsnyxb.com楊瑪麗,張保龍,郭佳茹,等.RNAi和GroEL介導(dǎo)的雙價(jià)轉(zhuǎn)基因番茄對(duì)TYLCV的抗性[J].江蘇農(nóng)業(yè)學(xué)報(bào),2014,30(5):1102鄄1108.doi:10.3969/j.issn.1000鄄4440.2014.05.027RNAi和GroEL介導(dǎo)的雙價(jià)轉(zhuǎn)基因番茄對(duì)TYLCV的抗性1221111楊瑪麗,搖張保龍,搖郭佳茹,搖趙統(tǒng)敏,搖余文貴,搖趙麗萍,搖王銀
2、磊(1.江蘇省農(nóng)業(yè)科學(xué)院蔬菜研究所,江蘇南京210014;2.江蘇省農(nóng)業(yè)科學(xué)院農(nóng)業(yè)生物技術(shù)研究所,江蘇南京210014)搖搖摘要:搖本研究將已構(gòu)建的SUC2鄄GroEL和pBI121鄄AC1鄄AC2植物表達(dá)載體共轉(zhuǎn)化番茄,共獲得19株卡那霉素抗性再生植株。經(jīng)過(guò)PCR和RT鄄PCR檢測(cè),初步獲得轉(zhuǎn)SUC2鄄GroEL載體的陽(yáng)性植株5株,轉(zhuǎn)pBI121鄄AC1鄄AC2載體的陽(yáng)性植株3株以及轉(zhuǎn)SUC2鄄GroEL/pBI121鄄AC1鄄AC2雙載體的陽(yáng)性植株2株。利用TYLCV侵染性克隆農(nóng)桿菌注射接種法對(duì)T代轉(zhuǎn)基
3、因陽(yáng)性植株進(jìn)行抗性鑒定,獲得抗TYLCV的轉(zhuǎn)基因植株7株。對(duì)T代未擴(kuò)出病毒條帶的T001代植株進(jìn)行抗性鑒定,結(jié)果發(fā)現(xiàn)轉(zhuǎn)SUC2鄄GroEL、pBI121鄄AC1鄄AC2、SUC2鄄GroEL/pBI121鄄AC1鄄AC2不同載體的株系植株中平均帶毒率依次是35%、25%和15%,推斷轉(zhuǎn)雙基因的植株的抗病能力優(yōu)于轉(zhuǎn)單個(gè)基因植株的抗病能力。關(guān)鍵詞:搖番茄黃化曲葉病毒;RNAi;GroEL;共轉(zhuǎn)化;轉(zhuǎn)基因番茄+中圖分類號(hào):搖S436.412.17搖搖搖文獻(xiàn)標(biāo)識(shí)碼:搖A搖搖搖文章編號(hào):搖1000鄄4440(2014
4、)05鄄1102鄄07RNAiandGroELmediatedresistancetotomatoyellowleafcurlvirus(TYLCV)intomato122111YANGMa鄄li,搖ZHANGBao鄄long,搖GUOJia鄄ru,搖ZHAOTong鄄min,搖YUWen鄄gui,搖ZHAOLi鄄ping,搖1WANGYin鄄lei(1.InstituteofVegetableCrops,JiangsuAcademyofAgriculturalSciences,Nanjing210014,
5、China;2.InstituteofAgro鄄biotechnology,JiangsuAcademyofAgriculturalSciences,Nanjing210014,China)搖搖Abstract:搖Tomatoyellowleafcurlvirus(TYLCV)isoneofthemostdevastatingvirusesofcultivatedtomatoesintheworld.Recently,thediseaseoccurredsuddenlyinChina,spreadingto
6、wardsnorthfromsouth,whichhasresultedingreateconomiclosses.ThelackofresistanceresourcelimitstheTYLCVresistancebreeding.Inordertogenerateengineeredre鄄sistance,theplantexpressionvectorsSUC2鄄GroELandpBI121鄄AC1鄄AC2wereco鄄transformedintotomatobyagrobacterium鄄med
7、iatedgenetictransformationmethod,andnineteenkanamycinresistantregeneratedtomatoplantswereobtained,inclu鄄dingfiveplantsofSUC2鄄GroELvector,threeplantsofpBI121鄄AC1鄄AC2vectorandthreeplantsofdoublevectorsconfirmedbyPCRandRT鄄PCR.Tgenerationofthetransgenicplantsw
8、ereinoculatedwithTYLCV,anditturnedout8wereTYLCV0resistant.ThecarryingratesofTYLCVbyTgeneration1收稿日期:2014鄄05鄄08achievedviavectorsSUC2鄄GroEL,pBI121鄄AC1鄄AC2,and基金項(xiàng)目:江蘇省自然科學(xué)基金項(xiàng)目(BK2011676);江蘇省農(nóng)業(yè)科技SUC2鄄GroEL/pBI12
