資源描述：
《棉仁植酸含量的離子色譜測(cè)定方法研究》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。
1、棉花學(xué)報(bào)CottonScience2015袁27淵6冤院561―569棉仁植酸含量的離子色譜測(cè)定方法研究陸智輝袁閻君袁辛文慧袁徐曉建袁祝水金袁陳進(jìn)紅*淵浙江大學(xué)農(nóng)業(yè)與生物技術(shù)學(xué)院農(nóng)學(xué)系/浙江省作物種質(zhì)資源重點(diǎn)實(shí)驗(yàn)室袁杭州310058冤摘要院為準(zhǔn)確測(cè)定棉仁植酸含量袁本研究從提取工藝堯色譜條件改進(jìn)等方面入手袁優(yōu)化建立了棉仁植酸含量的快速提取及離子色譜測(cè)定方法院棉仁粉樣品烘干后用乙醚脫脂袁以10mL0.66mol窯L－1的HCl提取袁沸水浴1h袁4℃震蕩12h袁離心分離袁取上清液稀釋并純化后上樣分析曰采用DIONEXICS-2000離子色譜儀堯
2、AG11-HCGuard(4mm伊50mm)保護(hù)柱和AG11-HCAnalytical(4mm伊250mm)分離柱袁在30.0mmol窯L－1的KOH為淋洗液袁流動(dòng)相流速為1.0mL窯min－1袁采樣時(shí)間為25min袁抑制電流為75mA袁柱溫30℃袁進(jìn)樣量20滋L的條件下進(jìn)行檢測(cè)遙結(jié)果表明袁棉仁植酸的回歸曲線2為0.999袁在檢測(cè)濃度內(nèi)線性關(guān)系良好曰各項(xiàng)指標(biāo)相對(duì)標(biāo)準(zhǔn)偏差均在5%以?xún)?nèi)袁平均回收率為98.64%～102.31%袁檢出限淵樣品中檢出待測(cè)物的最小濃度冤為0.059滋g窯mL－1袁定量限淵樣品中待測(cè)物能被定量測(cè)定的最低量冤為0.19
3、6滋g窯mL－1遙關(guān)鍵詞院棉仁曰植酸曰離子色譜中圖分類(lèi)號(hào)院O657.7+5∶S562文獻(xiàn)標(biāo)志碼院A文章編號(hào)院1002-7807淵2015冤06-0561-0910.11963/issn.1002-7807.201506008*LuZhihui,YanJun,XinWenhui,XuXiaojian,ZhuShuijin,ChenJinhong(310058,)Cottonseedmealisanimportantplantproteinresourceoffeedindustryandbreedingindustry,althoughit
4、containsmanyantinutritionalfactors.Phyticacid,asoneofthemainlyantinutritionalfactor,cannotbedigestedeasilybymonogastrican-imals,suchasfowlsandpigs,consequentlyresultsinthedecreasedbio-availabilityofmaterialelementsandleadstotheenvi-ronmentpollutionbythephytateinexcreta.Th
5、usfar,studyofbreedingcottonscontaininglowphyticacidgraduallybecomesthehotspot,andthemethodoffullyextractingphyticacidfromcottonseedsandprecisemeasurementisbecomingmoreandmoreimportantandnecessary.Anewmethodfortherapidextractionanddeterminationofphyticacidincottonseedkerne
6、lsbyionchromatographywasdevelopedinthisstudy.Afterthediethylethertreatmentofdrycottonseedkernelpowder,thesamplesweresuspendedin10ml0.66mol窯L－1HClandboiledfor1hour.Thenthesampleswereshakenat4℃for12hoursandcen-trifuged.Thesupernatantwasdilutedandpurifiedforloading.Toanalyze
7、thesample,DIONEXICS-2000ionchromatography,AG11-HCGuard(4mm伊50mm)protectioncolumnandAG11-HCGuard(4mm伊250mm)separationcolumnwereusedincontrolledconditions(washingbuffer:30.0mmol窯L－1KOH;reamspeed:1.0mL窯min－1;collectingtime:25min;75mAcur-rent;temperature:30℃;loadingamount:20滋
8、L).TheresultsshowedthatR2oflinearregressionis0.999,whichmeansthere-gressionrelationisgreat.Inadd
