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1、棉花學報CottonScience2013袁25淵2冤院178~183轉(zhuǎn)雙價基因棉花對根際土壤酶活性和養(yǎng)分含量的影響1,2223241*2*風春袁趙建寧袁李剛袁楊志國袁王慧袁吳冬梅袁紅雨袁楊殿林(1.內(nèi)蒙古師范大學生命科學與技術(shù)學院袁呼和浩特010022曰2.農(nóng)業(yè)部環(huán)境保護科研監(jiān)測所袁農(nóng)業(yè)部產(chǎn)地環(huán)境質(zhì)量重點實驗室/天津市農(nóng)業(yè)環(huán)境與農(nóng)產(chǎn)品安全重點開放實驗室袁天津300191曰3.山西農(nóng)業(yè)大學生命科學院袁山西太谷030801曰4.中國農(nóng)業(yè)科學院棉花研究所袁河南安陽455000)摘要院在田間試驗條件下袁以3種轉(zhuǎn)雙價基因棉和常規(guī)棉石遠321為研究對象袁比較分析轉(zhuǎn)
2、雙價基因棉和常規(guī)棉石遠321根際土壤酶活性及養(yǎng)分的變化遙結(jié)果表明袁轉(zhuǎn)雙價1Ac+基因棉sGK321與石遠321根際土壤速效磷和銨態(tài)氮含量無顯著差異袁而硝態(tài)氮含量則顯著高于石遠321曰轉(zhuǎn)雙價1Ac+2Ab基因棉淵雙抗蟲棉冤速效磷和銨態(tài)氮含量均顯著低于石遠321袁而硝態(tài)氮含量與石遠321無顯著差異曰轉(zhuǎn)雙價1Ac+基因棉淵抗蟲抗除草劑棉冤速效磷和硝態(tài)氮含量均顯著高于石遠321袁而銨態(tài)氮含量顯著低于石遠321遙sGK321棉與石遠321根際土壤脲酶堯堿性磷酸酶和過氧化氫酶活性均無顯著差異曰雙抗蟲棉土壤脲酶活性顯著低于石遠321袁堿性磷酸酶和過氧化氫酶活性與石遠3
3、21均無顯著差異曰抗蟲抗除草劑棉與石遠321土壤脲酶活性無顯著差異袁堿性磷酸酶活性顯著高于石遠321袁而過氧化氫酶活性顯著低于石遠321遙表明sGK321棉與石遠321根際土壤養(yǎng)分淵硝態(tài)氮除外冤含量和酶活性無顯著差異袁而雙抗蟲棉和抗蟲抗除草劑棉所呈現(xiàn)的差異是因不同品種所致遙關(guān)鍵詞院轉(zhuǎn)雙價基因棉曰根際土壤曰土壤酶活性曰土壤養(yǎng)分中圖分類號院S562.048文獻標志碼院A文章編號院1002-7807淵2013冤02-0178-061,2223241*FENGChun,ZHAOJian-ning,LIGang,YANGZhi-guo,WANGHui,WUDong
4、-mei,HONGYu,2*YANGDian-lin(1.,010022,;2.300191,;3.袁030801,;4.,455000,)Throughfieldexperiment,theenzymeactivityandnutrientcontentintherhizospheresoiloftransgeniccottonsGK321(1Ac+),transgeniccottonwithdoubleinsect-resistantgenes(1Ac+2Ab),transgeniccottonwithinsect-resistantandherbi
5、cide-resistantgenes(1Ac+)andnon-transgeniccottonwereanalyzed.TheresultsshowedthattheavailablephosphorusandammoniumnitrogenintherhizospheresoilhadnosignificantdifferencebetweentransgeniccottonsGK321andnon-transgeniccotton,whilethenitratenitrogensignificantlyhigherthannon-transgeni
6、ccotton.Theavailablephosphorusandammoniumnitrogenoftransgeniccottonwithdoubleinsect-resistantgeneswerebothsignificantlylowerthannon-transgeniccottonwhilethenitratenitrogenshowednodifference.Theavailablephosphorusandnitratenitrogenoftransgeniccottonwithinsect-resistantandherbicide
7、-resistantgeneswerebothsignificantlyhigherthannon-transgeniccotton,butnitratenitrogenwassignificantlylowerthannon-transgeniccotton.Theurease,alkalinephosphataseandcatalaseactivitiesintherhizospheresoil收稿日期院2012-09-18作者簡介院風春(1988-)袁女袁碩士研究生袁fengchun2006@163.com;*通訊作者院hongyu@imnu.ed
8、u.cn曰yangdianlin@caas.cn基金項目院轉(zhuǎn)基因生物新品種培育重
