轉(zhuǎn)BADH基因耐鹽堿玉米對(duì)根際土壤酶活性的影響.pdf

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1、第45卷第4期東北農(nóng)業(yè)大學(xué)學(xué)報(bào)45(4)：25-292014年4月JournalofNortheastAgriculturalUniversityApril2014網(wǎng)絡(luò)出版時(shí)間2014_4—2113：21：58【URL]http：／／www．cnki．net／kcms／detail／23．1391．S．20140421．1322．008．html轉(zhuǎn)BADH基因耐鹽堿玉米對(duì)根際土壤酶活性的影響邸宏，周成生，曾興，王術(shù)敏，王禹賀，王振華(東北農(nóng)業(yè)大學(xué)農(nóng)學(xué)院，哈爾濱150030)摘要：試驗(yàn)在田間種植條件下研究轉(zhuǎn)BADH基因耐鹽堿玉米在不同生育期對(duì)鹽堿土和普通耕

2、土中三種土壤酶活性的影響。結(jié)果表明，在整個(gè)生育期內(nèi)，兩種類(lèi)型土壤的土壤脲酶活性均呈現(xiàn)“升高一降低一升高”趨勢(shì)，蔗糖酶均呈現(xiàn)“升高一降低”趨勢(shì)，而脫氫酶在鹽堿土壤中呈現(xiàn)“升高一降低”趨勢(shì)，在普通耕土中始終是降低的；鹽堿土壤的脲酶和脫氫酶高于普通耕土，蔗糖酶活性低于普通耕土；與非轉(zhuǎn)基因受體對(duì)照相比，轉(zhuǎn)基因玉米株系種植在鹽堿土中促進(jìn)土壤脲酶活性，抑制土壤蔗糖酶活性，拔節(jié)期時(shí)達(dá)到差異顯著(P

3、抑制作用，分別在拔節(jié)期和吐絲期時(shí)達(dá)到差異顯著(P

4、ewithgeneBADH(ZeamaysL．)onsoilenzymesactivity[J]．JournalofNo~heastAgriculturalUniversity,2014，45(4)：25-29．(inChinesewithEnglishabstract)Effectofgeneticallymodifiedsalt-tolerantmaizewithgeneBADH(ZeamaysL．)onsoilenzymesactivity／DrHong，ZHOUChengsheng，ZENGXing，WANGShumin，WANGYuhe，WAN

5、GZhenhua(Sch0ol0fAgriculture，NortheastAgriculturalUniversity,Harbin150030，China)Abstract：Theefectsofgeneticallymodifiedsalt-tolerantmaizewiththegeneBADHonsoilurease，sucraseanddehydrogenaseactivityatdifferentgrowthstagesinsaline-alkalisoilandcommonsoilunderthefiledcondition．Theres

6、ultsshowedthatdudngthewholegrowthpedod，ureaseactivityincreasedatfirstandthendecreasedandincreasedfinallyintwotypesofsoil．Sucraseactivityincreasedatfirstandthendecreased，dehydrogenaseactivityincreasedatfirstandthendecreasedinsaline—alkalisoil，andalwaysdecreasedincommonsoil．Compare

7、dtocommonsoil，theureaseanddehydrogenaseactivityofsaline-alkalisoilwerehigherandsucroseactivitywaslower．Theplantingoftransgenicmaizeinsaline-alkalisoilincreased(26_77％)ureaseanddehydrogenaseactivityanddecreased(53．73％)sucroseactivitycomparedwithnon-transgenicmaize，andthedifference

8、sreachedthesignificantlevel(P<0．05)andth