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1、中國(guó)油料作物學(xué)報(bào)2014,36(3)：409—413ChineseJournalofOilCropSciencesdoi：10．7505／j．issn．1007—9084．2014．03．019轉(zhuǎn)基因大豆種植對(duì)根際土壤酶活性和養(yǎng)分的影響章秋艷，李剛，楊志國(guó)，王麗娟，王慧，常泓。，楊殿林，趙建寧(1．農(nóng)業(yè)部環(huán)境保護(hù)科研監(jiān)測(cè)所，天津，300191；2．山西農(nóng)業(yè)大學(xué)生命科學(xué)院，山西太谷，030801；3．內(nèi)蒙古師范大學(xué)生命科學(xué)與技術(shù)學(xué)院，內(nèi)蒙古呼和浩特，010022)摘要：采用盆栽試驗(yàn)，以耐草甘膦大豆M88、抗蟲耐草甘膦大豆zB及常規(guī)大豆中黃13為研究對(duì)
2、象，比較分析轉(zhuǎn)基因大豆對(duì)根際土壤酶活性和養(yǎng)分含量的影響。結(jié)果表明，在大豆成熟期時(shí)，與常規(guī)大豆中黃13相比，M88、ZB根際土壤堿性磷酸酶活性、過氧化氫酶活性、速效磷含量無顯著差異，硝態(tài)氮含量顯著下降。根際土壤脲酶活性、銨態(tài)氮含量則表現(xiàn)不同，其變化隨大豆品種的不同而不同。相較于常規(guī)大豆中黃13，M88根際土壤脲酶活性和銨態(tài)氮含量無顯著差異；ZB根際土壤脲酶活性顯著下降，而銨態(tài)氮含量則顯著上升。關(guān)鍵詞：轉(zhuǎn)基因大豆；CP4epsps耐草甘膦基因；Btc~lAb抗蟲基因；酶活性；氮含量中圖分類號(hào)：$565．1文獻(xiàn)標(biāo)識(shí)碼：A文章編號(hào)：1007—9084(20
3、14)03—0409—05EfectsoftransgenicsoybeanonenzymeactivitiesandnutrientsinrhizospheresoilZHANGQiu—yan，LIGang，YANGZhi—guo，WANGLi—juan，WANGHui，CHANGHong，YANGDian—lin，ZHAOJian—ning(1．Agro—EnvironmentalProtectionInstitute，Min~tryofAgriculture，Tianfin300191，China；2．CollegeofLifeScience
4、，ShanxiAgriculturalUniversi秒，030801，China；3．CollegeofLifeScienceandTechnology，InnerMongoliaNormalUnivemity，Hohhot010022，China)Abstract：Transgeniccropsweregrowninanincreasinglylargescalearoundwodd，andaccompaniedbypub-licconcelTlabouttheirpotentialecologicalandenvironmentalimpac
5、ts．Evaluationoftheeffectofgeneticallymodi—fledcropsonsoilenzymeactivityandnutrientcontentiscriticalforthesoilecosystem．Inordertoinvestigatetheeffectsofplantingtransgenicsoybeanonenzymeactivitiesandnutrientcontentsofrhizospheresoil，thechangesoftheenzymeactivities(urease，alkalin
6、ephosphataseandhydrogenperoxidase)andnutrientcontents(nitratenitro—gen，ammoniumnitrogen，availablephosphorus)inrhizospheresoilwereexaminedinmicrocosmexperiments．TwovarietiesoftransgenicsoybeanM88(CP4印)，ZB(BtcrylAb+CP4epsps)andaconventionalsoybeanZhong—huang13wereselected．Compar
7、edwiththeconventionalvariety，thesoilenzymeactivities(alkalinephosphataseandhydrogenperoxidase)andavailablephosphoruscontentoftransgenicsoybeanhadnosignificantdifference，butthenitratecontentdecreasedsignificantly．However，remarkabledifferencesexistedbetweenM88andZBintermsofammon
8、iumnitrogenandureaseactivityinrhizospheresoil．Comparedwiththe
