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1、行政院農(nóng)業(yè)委員會(huì)水產(chǎn)試驗(yàn)所黃培安褐藻醣膠(Fucoidan)對(duì)於腫瘤細(xì)胞之抑制作用WhatisFucoidan？！Thepolysaccharidewasnamedas“fucoidin”whenitwasfirstisolatedfrommarinebrownalgaebyKylinin1913.butsomealsocalleditfucan,fucosanorsulfatedfucan.Nowitisnamedas“fucoidan”accordingtoIUPACrules.Fucoidansaresulfatedpolysaccharidesextracte
2、dfrommarinebrownseaweed.(Matouetal.,2002)Fucoidanismajorcomposingoffucose,uronicacid,galactose,xyloseandsulphatedfucose.(MaraisandJoseleau,2001)Thechemicalcompositionsofmostfucoidansarecomplex.Thestructuresoffucoidansfromdifferentbrownalgaevaryfromspeciestospecies.(Lietal.,2008)Fucoidan
3、containsasubstantialpercentageofL-fucoseandsulfateestergroups,andthestructuralcharacteristicsoffucoidanareverysimilartothoseofheparin.(Yangetal.,2006)Comparedwithothersulfatedpolysaccharides,fucoidansarewidelyavailablefromvariouskindsofcheapsources,somoreandmorefucoidanshavebeeninvestig
4、atedinrecentyearstodevelopthedrugsorfunctionalfoods.(Lietal.,2008)WhereisFucoidanfrom？！WhatwehaveinTaiwan？！Method3.Method2.Method1.Active150KDa-50KDa50KDa-10KDa10KDa-500Da<500DaMethod1.6.1％（135KDa）92.3％（9KDa）Method2.5.0％（137KDa）7.6％（15KDa）32.7％（6KDa）54.0％（<500Da）Method3.2.1％（145KDa）3.8％
5、（7KDa）94.1％（<500Da）RetentiontimeArea19'0662.29glucose210'08181.94galactose310'87335.06fucose1.「誘導(dǎo)癌細(xì)胞凋亡（Apoptosis）」2.「活化免疫作用」3.「抑制血管新生作用」ANTI-TUMOR?!（A）（B）圖、Fucoidan處理人類肝癌細(xì)胞之細(xì)胞型態(tài)變化，箭頭處為細(xì)胞型態(tài)不完整。（A）控制組、（B）Fucoidan。Fucoidan(mg/ml)「1.誘導(dǎo)癌細(xì)胞凋亡（Apoptosis）」圖、Fucoidan對(duì)於人類肝癌細(xì)胞株HepG2細(xì)胞週期之影響。(A)medi
6、um(B)1mg/ml(C)2.5mg/ml(D)5mg/ml。4.8%39.5%54.2%56.8%在細(xì)胞週期中：G1時(shí)期就是細(xì)胞開始休息。S時(shí)期，DNA開始準(zhǔn)備合成。M時(shí)期則準(zhǔn)備分裂。將細(xì)胞週期停留在Sub-G1時(shí)期，則細(xì)胞不會(huì)繼續(xù)分裂，而沒法增殖，終至凋亡。凋亡晚期凋亡早期圖、磷脂絲氨酸(PS)外翻(AnnexinV-FITC/PI雙染法)分析Fucoidan對(duì)於人類肝癌細(xì)胞株HepG2誘導(dǎo)之細(xì)胞凋亡。(A)medium(B)1mg/ml(C)2.5mg/ml(D)5mg/ml。圖、Fucoidan對(duì)於人類肝癌細(xì)胞株HepG2對(duì)於人類肝癌細(xì)胞株HepG2casp
7、ases活性之影響。(A)medium(B)1mg/ml(C)2.5mg/ml(D)5mg/ml。Caspases引發(fā)一連串蛋白質(zhì)酶解作用，傳遞死亡訊息給下游酵素，進(jìn)一步造成DNA的片段化。圖、Fucoidan對(duì)於人類肝癌細(xì)胞株HepG2DNAladder之影響DNA已被切成小片段，因此在DNA凝膠分析時(shí)，可以觀察到DNA階層的現(xiàn)象?！?.活化免疫作用」圖、Fucoidan對(duì)於免疫細(xì)胞HB4C5之影響圖、Fucoidan對(duì)於免疫細(xì)胞HB4C5分泌IgM抗體之影響＊＊＊＊＊圖、Fucoidan對(duì)於吞噬細(xì)胞J774.1之影響圖、Fucoidan對(duì)於吞噬細(xì)
