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《結(jié)球白菜下胚軸原生質(zhì)體培養(yǎng)及其體細(xì)胞胚植株再生》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。
1、植物學(xué)通報(bào)2006,23(3):275~280ChineseBulletinofBotany.組織培養(yǎng)簡(jiǎn)訊.結(jié)球白菜下胚軸原生質(zhì)體培養(yǎng)及其體細(xì)胞胚植株再生1*12劉凡趙泓秦帆(1國(guó)家蔬菜工程技術(shù)研究中心北京100089)(2四川省農(nóng)業(yè)科學(xué)院園藝研究所成都610066)摘要結(jié)球白菜(Brassicacampestrisssp.pekinensis)的原生質(zhì)體培養(yǎng)由于基因型依賴性強(qiáng),細(xì)胞易褐化,愈傷組織的芽誘導(dǎo)率低等而難于再生植株。本實(shí)驗(yàn)以結(jié)球白菜的下胚軸原生質(zhì)體為試材,研究了影響其細(xì)胞分裂及愈傷組織形成的因素,探索了經(jīng)過體細(xì)胞胚發(fā)生途徑獲得再生植株
2、的技術(shù)。結(jié)果表明,試材的基因型及培養(yǎng)基組成影響細(xì)胞分裂及褐化;KM8P是結(jié)球白菜原生質(zhì)體培養(yǎng)更適宜的培養(yǎng)基,能顯著減輕細(xì)胞的褐化;液體培養(yǎng)基中一定濃度的活性炭能在一定程度上減輕細(xì)胞褐化進(jìn)程,并有利于星狀細(xì)胞團(tuán)的形成;基因型Asko中,愈傷組織形成體細(xì)胞胚的結(jié)構(gòu),其發(fā)生的頻率約為5%,該類體細(xì)胞胚能全部順利地發(fā)育成完整植株。本技術(shù)具有再生植株形成容易、頻率較高且通過體細(xì)胞胚發(fā)生途徑等優(yōu)點(diǎn)。關(guān)鍵詞結(jié)球白菜,體細(xì)胞胚,原生質(zhì)體培養(yǎng),植株再生TheProtoplastCultureofBrassicacampestrisssp.pekinensisand
3、PlantRegenerationviaSomaticEmbryogenesis1*12FanLiu,HongZhao,FanQin(1NationalEngineeringResearchCenterforVegetables,Beijing100089)(2InstituteofHorticulture,SichuanAcademyofAgriculturalScience,Chengdu610066)AbstractSomefactorsblockingtheprotoplastregenerationofBrassicacampestri
4、sssp.pekinensisincludegenotype-dependentcellbrowningandlowfrequencyofshootinduction.Screeningforagenotypethatiseasytocultureandestablishinganexperimentalsystemforefficientshootinduc-tionwillgreatlybenefitthegeneticinnovationofB.campestrisssp.pekinensisviasomatichybridization.
5、HypocotylprotoplastsofseveralvarietiesofB.campestrisssp.pekinensiswerecultured,andfactorsinfluencingcelldivisionandbrowningwerestudied.Celldivisionandbrown-ingwereinfluencedheavilybygenotypesofmaterialsandtheculturemediaused.KM8Pmediumeffectivelyreducedthebrowningtendencyofth
6、ecellsandwasagoodmediumforprotoplastcultureofB.campestrisssp.pekinensis.Activatedcharcoalhadaspecificfunctionofreducingcellbrowningandpromotingthestar-shapedmicrocalliformation.Thesomaticembryoswere-1observedoncalliofthegenotypeAskoinshoot-inducingmediumcontaining3mg.LAgNO3,w
7、ithafrequencyofabout5%.Intactplantsweredevelopedeasilyfromtheembryos.Thisproce-durehasadvantagesineasilyregeneratingplantswithrelativelyhighfrequencyviaasomaticembryogenesispathway.KeywordsBrassicacampestrisssp.pekinensis,somaticembryogenesis,protoplastculture,plantregenerati
8、on收稿日期:2005-12-22;接受日期:2006-03-09基金項(xiàng)目:北京市自然科學(xué)基金(5050001)和北京市科技發(fā)展計(jì)劃項(xiàng)目
