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1、第6期華東師范大學(xué)學(xué)報(bào)(自然科學(xué)版)No.62006年11月JournalofEastChinaNormalUniversity(NaturalScience)Nov.2006文章編號(hào):100025641(2006)0620053206黑質(zhì)注射魚藤酮對(duì)大鼠行為和丘腦VL核自發(fā)放電的影響張貝,何德富,東春陽,盧湘岳(華東師范大學(xué)環(huán)境科學(xué)系,上海200062)摘要:研究中腦黑質(zhì)注射魚藤酮對(duì)大鼠行為和丘腦VL核神經(jīng)元電活動(dòng)的影響.運(yùn)用網(wǎng)格、斜板和開闊試驗(yàn)檢測(cè)大鼠行為改變;微電極記錄丘腦VL核神經(jīng)元自發(fā)電活動(dòng).魚藤
2、酮腦內(nèi)注射28d后,大鼠在網(wǎng)格上的移動(dòng)潛伏期明顯增長,沿斜板下滑次數(shù)顯著增多,自主活動(dòng)減少,而靜止性蹲坐時(shí)間顯著增多;丘腦VL核神經(jīng)元自發(fā)放電頻數(shù)顯著降低,電頻譜峰向低頻區(qū)聚集,Ⅰ型簇狀放電型式減少,更多的表現(xiàn)為規(guī)律型放電或Ⅱ型簇狀放電.結(jié)果表明黑質(zhì)注射魚藤酮能誘發(fā)大鼠出現(xiàn)肌僵直、行動(dòng)遲緩、震顫等類帕金森病征狀,丘腦VL核神經(jīng)元自發(fā)放電活動(dòng)相應(yīng)減弱,放電型式有所改變.關(guān)鍵詞:魚藤酮;黑質(zhì);自發(fā)放電;行為;丘腦腹外側(cè)核;頻譜中圖分類號(hào):Q948文獻(xiàn)標(biāo)識(shí)碼:AEffectsofMicroinjectingRot
3、enoneintoSNcontheBehaviorandSpontaneousDischargeofVentrolateralThalamusinRatsZHANGBei,HEDe2fu,DongChun2yang,LUXiang2yue(DepartmentofEnvironmentalScience,EastChinaNormalUniversity,Shanghai200062,China)Abstract:TheeffectsofmicroinjectingrotenoneintoSNcontheb
4、ehaviorandspontaneousac2tivitiesoftheventrolateralthalamusneuronsinratswerestudied.28daysaftertheinjectionofrotenone,boththelatencyofrats′movingonvertical2gridandthenumbersofrats′slidingdownthedeclined2planesignificantlyincreased.Thewillfulmovementsofanima
5、lsweresignificantlydecreased,andthetimeofinactivesittingwassignificantlyincreased.ThespontaneousdischargerateoftheVLneuronssignificantlydecreased.Neuronswithtype2Ⅰburstingfiredecreased,however,theregularfireandtype2Ⅱburstingfireincreased.Thepeaksofthepower
6、spectrumofneuronsmovedtothelowfrequencyarea.TheresultssuggestthatmicroinjectingrotenoneintoSNccouldcausesymptomssimilartoParkinsonismsyndromesuchasrigidity,slowmoving,andtremoretc.Theresultsalsoindicatethatitalsocausesthedecreaseofspontaneouselectroactivi2
7、tiesofVLneuronsandthechangesoftheirfiringpatterns.收稿日期:2006208第一作者:張貝(1982-),女,碩士研究生;E2mail:breetty96@163.com.通訊作者:何德富(1969-),男,博士,講師;E2mail:dfhe@des.ecnu.edu.cn.54華東師范大學(xué)學(xué)報(bào)(自然科學(xué)版)2006年Keywords:rotenone;substentianigra;spontaneousdischarge;behavior;ventrola
8、teralthalamus;powerspectrum0引言帕金森病(Parkinson′sdisease,PD)的病因?qū)W研究表明:農(nóng)藥接觸史是誘發(fā)PD的一個(gè)[1,2][3]重要的危險(xiǎn)因素.Ferrant等采用靜脈慢性注射魚藤酮(rotenone)的方法,誘發(fā)動(dòng)物明顯的運(yùn)動(dòng)障礙和僵直.魚藤酮對(duì)大鼠黑質(zhì)致密部(substentianigracompact,SNc)多巴胺能[4]神經(jīng)元有選擇性損傷作用,據(jù)此有理由推測(cè)
