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1、結(jié)直腸癌中抑癌基因ING1表達探究作者：張森韋建寶周永淳陳利生【摘要】目的探討抑癌基因ING1及其蛋白p33/INGl在結(jié)直腸癌中的表達。方法用RTPCR檢測35例散發(fā)性結(jié)直腸癌中ING1mRNA的表達水平，并應(yīng)用SP法檢測60例散發(fā)性結(jié)直腸癌及正常黏膜組織中P33/ING1蛋白的表達。結(jié)果結(jié)直腸癌組織、正常黏膜組織中P33/ING1蛋白陽性表達率分別為43.3%(26/60)、100%(60/60),兩者比較差異有統(tǒng)計學意義(P<；0.01)op33/INGl在無淋巴結(jié)轉(zhuǎn)移組及淋巴結(jié)轉(zhuǎn)移組中的陽性
2、表達率分別為57.6%(19/33)、25.9%(7/27),兩者比較差異有統(tǒng)計學意義(P<；0.05)o在DukesA.B期和DukesC、D期患者癌組織中p33/ING1的陽性表達率分別為56.7%(17/30)、30.0%(9/30),兩者比較差異有統(tǒng)計學意義(P<；0.05)o結(jié)論ING1及p33/ING1蛋白的低表達與散發(fā)性結(jié)直腸癌發(fā)生、發(fā)展密切相關(guān)，臨床分期越差者表達水平越低。【關(guān)鍵詞】抑癌基因ING1結(jié)腸腫瘤直腸腫瘤[Abstract]ObjectiveTodiscusstheex
3、pressionoftumorsuppressorgeneING1anditsproteinp33/INGlindevelopmentofsporadiccolorectaicarcinoma.MethodsReversetranscriptionpolymerasechainreactionwasusedtodetectmRNAexpressionlevelofING1in35caseswithsporadiccolorectalcarcinomaandstreptomyceteavidinperox
4、idaseimmunohistochemistrytomeasureexpressionlevelofproteinp33/INGlincolorectaicanceroustissuesandnormalmucoustissuesfrom60caseswithsporadiccolorectaicarcinoma.ResuItsExpressionlevelofp33/INGlmRNAincolorectaicanceroustissueswasmoresignificantlydecreasedth
5、anthatinnormalmucoustissues?Expressionlevelofp33/INGlmRNAinthetumorsofpatientsatstageofDukesAandBwassignificantlyhigherthanthatinpatientsatstageofDukesCandD,asindicatedthattheexpressiondecreasedwithpoorclinicalstage.Positiveexpressionrotesofp33/INGlincol
6、orectaicanceroustissuesandnormalmucoustissueswere43.3%(26/60)and100%(60/60),respectively,withsignificantstatisticaldifference(P&It；0.01)?Positiveexpressionratesofp33/INGlingroupswithorwithoutlymphnodemetastasiswere25.9%(7/27)and57.6%(19/33)respectively(P
7、<；0.05).Positiveexpressionratesofp33/INGlinpatientsatstageofDukesA,BandatstageofDukesCandDwere56.7%(17/30)and30.0%(9/30)respectively(P<；0.05).ConclusionsThedecreasedexpressionofp33/INGlandlowexpressionofproteinp33/INGlarecloselyrelatedwithdevelopment
8、ofsporadiccolorectaicarcinoma?[Keywords]Tumorsuppressorgene；Inhibitorofgrowth；Coloncancer；Rectaicancer結(jié)直腸癌是臨床上一種常見的惡性腫瘤。隨著分子生物學研究的進展，結(jié)直腸癌的發(fā)生被認為與癌基因的功能增強、抑癌基因功能低下以及DNA錯配修復(fù)基因缺失等因素密切相關(guān)。ING1(inhibitorofgrowth)基因是近年來克隆出來的一個抑癌基因
