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1、南方農(nóng)業(yè)學報JournalofSouthernAgrieuhure2014,45(7):1160—1165ISSN2095—1191;CODENNNXAABDOI:10.3969/j:issn.2095—1191.2014.7.1160桃肉色、果皮茸毛性狀的SSR標記篩選張妤艷,馬瑞娟,俞明亮,宋宏峰(江蘇省農(nóng)業(yè)科學院園藝研究所,南京210014)摘要:【目的】研究與桃果肉顏色和果皮茸毛性狀相關(guān)的SSR標記,為桃早期分子標記輔助育種提供參考依據(jù)。【方法】以油蟠桃98—5—8與普通桃91—42—51雜交
2、的F。分離群體74個單株為材料,對其果實白肉/黃肉、有毛/無毛兩個性狀進行SSR分子標記?!窘Y(jié)果】在66對引物中,引物UDP96—005和pchgms3、UDP97—401和CPSCT030分別在白肉/黃肉單株、有毛/無毛單株及混合基因池間擴增出差異條帶。表型性狀與標記連鎖結(jié)果表明,SSR標記UDP96—005(164bp)與白肉性狀連鎖,遺傳距離為4.06cM;SSR標記CPSCT030(191bp)與有毛性狀連鎖,遺傳距離為8.2cM。對20個桃栽培品種進行驗證,UDP96—005標記對白、黃肉品
3、種的檢測符合率為85%,CPSCT030標記對有無茸毛品種的檢測符合率為50%?!窘Y(jié)論】篩選獲得分別與桃白肉、有毛性狀連鎖的ssR標記UDP96—005與CPSCT030,可用于桃分子標記輔助育種,并為發(fā)掘與桃果肉顏色和茸毛性狀相關(guān)基因奠定基礎(chǔ)。關(guān)鍵詞:桃;肉色;果皮茸毛;SSR標記;分子標記輔助育種中圖分類號:$662.1文獻標志碼:A文章編號:2095—1191(2014)07—1160—06ScreeningonSSRmarkerslinkedtofleshcolourgeneandnectar
4、inegeneofPrunuspersica(L.)BatschZHANGYu—yan,MARui-juan,YUMing-liang,SONGHong—feng(InstituteofHorticuhure,JiangsuAcademyofAgriculturalSciences,Nanjing210014,China)Abstract:【Objective】TheSSRnlarkerslinkedtofleshcolourgeneandnectarinegeneofpeachwerestudied
5、inordertoprovidereterencesformolecularassistedselection(MAS)inpeachbreeding.【Method]TheF1segregationpopula—tion,totalof74seedlings(1一year—old)derivedfromcrossflatarine(PnJHUSpezsicaL.)98—5—8xpeach(P.pericaL.)91—42—51wastestedforidentit}ingSSRmolecularnl
6、arkerslinkedtofleshcolorandpeelfuZZbybulkedsegregantanalysis(BSA)method.【Result】Outof66pairsofprimers,twoprimersUDP96—005andpchgms3couldamplify-diflerentbandsbetweenbulkedgenepoolofwhitefleshandyellowfleshvarietiesandmixedgenepool,whichindi—catedthatthe
7、nlarkersshouldbelinkedtofleshcolorcharacter.TwoprimersUDP97—401andCPSCT030couldanl—plifvdiflerentbandsbetweenbulkdedgenepoolofpeachandnectarinevarietiesandmixedgenepool,whichindicatedthattheinarkersshouldbelinkedtopeelfuZZtrait.Fromtheresultsofphenotypi
8、ctraitswithnlarkerlinkage,thenearestnlarker,UDP96—005(164bp)waslinkedtowhitefleshatadistanceof4.06cM,CPSCT030waslinkedtoDubescentpeelatadistanceof8.2cM.Bvverificationresultsof20peachcuhivars,UDP96—005nlarkerwaseffi—cientwithascor