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1、南方農(nóng)業(yè)學(xué)報JournalofSouthernAgricultureISSN2095—1191;CODENNNXAABhttp://www.nfnyxb.cnDOI:10.3969/j:issn.2095-1191.2015.1.36外源亞精胺對澇脅迫下玉米幼苗根的氧化傷害緩解效應(yīng)程明明1’2,杜紅陽2,劉懷攀2+(1鄭州大學(xué)生命科學(xué)學(xué)院,鄭州450001;2周口師范學(xué)院植物遺傳與分子育種重點實驗室,河南周口466001)摘要:【目的】研究亞精胺對澇脅迫下玉米幼苗根系抗氧化代謝酶活性及膜質(zhì)過氧化產(chǎn)物的影響,為多胺的農(nóng)業(yè)實踐應(yīng)用提供理論依據(jù)。【方法】以兩個抗?jié)承圆煌挠衩灼贩N(鄭單
2、958,抗?jié)承暂^弱;浚單22,抗?jié)承暂^強)為材料,設(shè)置正常供水、澇脅迫和澇脅迫十lmmol/L亞精胺3個處理,處理6d后取幼苗根測定其抗氧化代謝酶活性及膜質(zhì)過氧化產(chǎn)物含量?!窘Y(jié)果】澇脅迫下,兩個品種玉米幼苗根系抗氧化酶活性、幼苗相對干物質(zhì)增長速率(I①wm)和根系活力均下降,相對電解質(zhì)滲漏率(RELR)、超氧陰離子(0f)產(chǎn)生速率和丙二醛(MDA)含量上升,且對抗?jié)承暂^弱的鄭單958影響較為明顯。施加亞精胺后,明顯提高了澇脅迫下抗?jié)承暂^弱的鄭單958幼苗根系的超氧化物歧化酶(SOD)、過氧化物酶(POD)、過氧化氫酶(CAT)和抗壞血酸氧化酶(APX)活性、RDWIR和根系活力,
3、降低TRELR、Ⅸ產(chǎn)生速率和MDA含量;與澇脅迫相比,其SOD、POD、CAT和APX活性、RDWIR和根系活力分別上升了46.4%、50.O%、96。0%、31.o%、33.5%和73.8%,而RELR、暉產(chǎn)生速率和MDA含量分別下降了28.4%、33.6%和19.4%?!窘Y(jié)論】適當(dāng)濃度的亞精胺能通過提高玉米幼苗根系中抗氧化代謝酶活性來降低0f的產(chǎn)生速率和MDA積累量,緩解澇脅迫對玉米根系質(zhì)膜的傷害,從而提高玉米幼苗根系活力,促進玉米幼苗生長。關(guān)鍵詞:亞精胺;玉米幼苗;抗氧化酶;澇脅迫中圖分類號:Q945.78文獻標(biāo)志碼:A文章編號:2095—1191(2015)01—0036
4、—06Mitigatingeffectsofexogenousspermidineonoxidativeinjury‘一’●·●·‘Jn●l’‘inducedbywaterlogglngstressintherootS0tmaizeseedlingsCHENGMing—ming1”,DUHong—yan92,LIUHuai-pan24(1SchoolofLifeSciences,ZhengzhouUniversitv,Zhengzhou450001,China;2KevLaboratoryofPlantGeneticsandMolecularBreeding,ZhoukonNor
5、malUniversity,Zhoukon,Henan466001,China)Abstract:【Objective】Inordertoprovidetheoreticalbasisfortheagriculturalpracticeapplicationofpolyamines,theeffectsofexogenousspermidine(Spd)ontheantioxidantenzymeactivitiesandmetabolitecontentsinmaizeseedlingrootswerestudiedunderwaterloggingstress.【Method
6、]Twomaizecultivars,Zhengdan958(waterlogging—sensitive)andJundan22(waterlogging-tolerant),wereusedasexperimentmaterials.Threetreatmentswereimplemented,namelythecontrol,waterloggingandwaterlogging+Spd(1mmol/L).Aftertreatingforsixdays,theantioxidantenzymeactivitiesandmetabolitecontentsinmaizesee
7、dlingrootsweredetected.【Resuh】Underwaterloggingstress,theactivitiesoftheseen—zymes(supemxidedismutase:SOD,peroxidase:POD,catalase:CAT,andascorbicacidoxidase:APX),relativedroughtweightincreaserate(RDWIR)ofseedlingandrootvigourdecreased.Relativ