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1、齊齊哈爾醫(yī)學(xué)院學(xué)報(bào)2015年第36卷第l8期JournalofOiqiharUniversityofMedicine,2015,Vo1.36。No.18.經(jīng)驗(yàn)交流.川崎病患Jk~f,周血淋巴細(xì)胞亞群的變化及臨床診斷意義郭曉軍盧敏君占瓊英【摘要】目的探究川崎病患JL~'b周血淋巴細(xì)胞亞群的變化情況及其臨床診斷價(jià)值。方法選取我院2012年6月至2014年9月收治的25例川崎病患兒為實(shí)驗(yàn)組,另選取同期來(lái)我院體查的l5例健康體檢兒童為對(duì)照組,采用流式細(xì)胞術(shù),對(duì)兩組兒童外周血淋巴細(xì)胞亞群(CD3、CD3CD8、CD3CD4、CD3一CD16CD56、CD19CD23、CD3一CD19、
2、CD4CD25+、CD4/CD8)進(jìn)行檢測(cè),觀察兩組檢測(cè)結(jié)果。結(jié)果實(shí)驗(yàn)組CD4/CD8、CD3CD4、CD19CD23、CD3一CD19明顯高于對(duì)照組,CD3CD8、CD3、CD4CD25呈下降趨勢(shì),差異有統(tǒng)計(jì)學(xué)意義(P<0.05);CD3、CD3CD8、CD3CD4、CD19CD23、CD3一CD19、CD4CD25、CD4/CD8的ROC曲線下面積依次為76.0%、80.5%、72.4%、73.6%、79.6%、72.7%、73.6%。結(jié)論兒童川崎病急性期往往伴有免疫紊亂及免疫活化情況,可將CD4/CD8、CD3一CD19、CD3CD8、CD3CD4的診斷界值作為JiI崎
3、病的早期診斷指標(biāo),值得臨床積極借鑒。【關(guān)鍵詞】川崎??;淋巴細(xì)胞亞群;診斷價(jià)值ChangesandclinicalsignificanceofdiagnosisofperipheralbloodlymphocytesubsetsonchildrenkawasakidiseaseGUOXiaojun,eta1.DepartmentofPediatrics,TangxiaHospital,Dongguan,Guangdong523710.ina.【Abstract】ObjectiveToexplorethevalueofchangesofperipheralbloodlymphoc
4、ytesubsetsinchildrenwithKawasakidiseaseandclinicaldiagnosis.Methods25cesesofKawasakidiseasepatientsadmittedinourhospitalfromJune2O12toSeptember2014astheexperimentalgroup.theother15casesofhealthychildrenwereselectedinourhospitalwhodidphysicalexaminationduringtllesameperiodasthecontrolgroup.u
5、singflowcytometry,detectedtwogroupsofperipheralbloodlymphocytesubsets(CD3、CD3CD8、CD3CD4、CD3一CDl6CD56、CD19CD23、CD3一CD19、CD4CD25、CIM/CD8),wereobservedthedetectionresultinthetwogroups.ResultsInexperimentalgroupCD4/CD8(%:2.1±0.7vs1.6±0.5),CD3CD4(%:34.8±8.1vs18.9±4.8),CD19CD23(%:15.2±6.1vs10.6±4
6、.7),CD3一CD19’(%:34.9±12.8vs22.9±6.9)weresignificantlyhigherthanthecontrolgroup,CD3CD8(%:6.9±4.9vs23.0±5.2),CD3(%:55.1±11.1vs64.8±5.7).CD4CD25(%:4.8±1.1vs6.2±1.7)decreased,thediferenceswerestatisticallysignificant(P<0.05):ROCcurveareaofCD3,CD3CD8,CD3CD4,CD19CD23,CD3一CD19.CD4CD25.CD4/CD8were7
7、6%,80.5%,72.4%,73.6%,79.6%,72.7%.73.6%.ConclusionsChildrenKawasakidiseaseoftenassociatedwithimmunedisordersandimmuneactivation.thevalueofCD4/CD8,CD3一CD19,CD3CD8,CD3CD4canbeusedasanearlydiagnosticindexesofKawasakidisease.isworthyofclinicalreferenceactivel