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1、熱帶作物學(xué)報(bào)2012，33(3)：446—452ChineseJournalofTropicalCrops大豆GmPic基因的分離及表達(dá)分析吳輝，符秀梅，張巧玲，郭倩麗，陳銀華，沙愛華1海南大學(xué)農(nóng)學(xué)院海南省熱帶生物資源可持續(xù)利用重點(diǎn)實(shí)驗(yàn)室．海南?？?702282中國(guó)農(nóng)業(yè)科學(xué)院油料作物研究所．湖北武漢430062摘要對(duì)光敏感型大豆品種“中豆24”進(jìn)行長(zhǎng)13、短日和自然光3種光周期處理．采用cDNA—AFLP的方法篩選差異片段，并進(jìn)一步通過RACE技術(shù)分離了該基因。該基因全長(zhǎng)983bp，包含一個(gè)完整的開放閱讀框，編碼248個(gè)氨基酸，在遺傳關(guān)系上與線粒體磷酸鹽轉(zhuǎn)運(yùn)蛋白高度同源。通過
2、半定量RT—PCR對(duì)該基因在不同光周期中的表達(dá)模式進(jìn)行分析，結(jié)果表明，該基因在大豆生長(zhǎng)發(fā)育的早期階段表達(dá)．短日照抑制其表達(dá)，而長(zhǎng)日照則增強(qiáng)其表達(dá)。因此，大豆線粒體磷酸鹽轉(zhuǎn)運(yùn)蛋白基因可能是作為一種負(fù)調(diào)控因子參與大豆光周期反應(yīng)。對(duì)大豆線粒體磷酸鹽轉(zhuǎn)運(yùn)蛋白基因的研究能為進(jìn)一步闡明大豆光周期反應(yīng)分子機(jī)理打下基礎(chǔ)關(guān)鍵詞大豆；線粒體磷酸鹽轉(zhuǎn)運(yùn)蛋白；光周期；cDNA—AFLP中圖分類號(hào)$565．1：Q781：Q786文獻(xiàn)標(biāo)識(shí)碼ACloningofthePhotoperiodResponseGeneGmPicfromSoybeanandExpressionAnalysisWUHui，F(xiàn)UXi
3、umei，ZHANGQiaoling，GU0Qianli，CHENYinhua，SHAAihua1HainanKeyLaboratoryforSustainableUtilizationofTropicalBioresource，HainanUniversity,Haikou，Hainan570228,China2InstituteofOilCropsResearch，ChineseAcademyofAgricultureSciences，Wuhan,Hubei430062，ChinaAbstractInthisstudy，thelight—sensitivesoybeanv
4、ariety“ZhongDou24”wasprocessedforalongday，shortdayphotoperiodandnaturallight，andthenthediferentialexpressionfragmentswerescreenedviacDNA-AFLP．ThefulllengthofdifferentialgeneGmPicwasobtainedbyRACEtechnology．ThegeneGmPicis983bp，whichcontainsacompleteopenreadingflameencoding248aminoacids．Thege
5、neinthegeneticrelationshipishighlyhomologytothemitoehondrialphosphatetransportproteinaminoacids．Finally，expressionprofileofthegeneGmPicinleavestreatedwithdifferentphotoperiodswascarriedoutbysemi—quantitativeRT—PCR．TheexpressionpatternanalysisshowedthattheexpressionofthegeneGmPicisinhibitedb
6、yshort—dayinanearlystageofsoybean"sgrowthanddevelopment；whileitisenhancedbylong-day．SothegeneGmPicofsoybeanmitochondrialphosphatetransportergenemayserveasanegativeregulatorofphotoperiodicresponseinsoybean．Thisstudyofsoybeanmitochondrialphosphatetransportergenewasconductedtofurtherclarifythe
7、molecularmechanismofsoybeanphotoperiodresponse．keywordsSoybean(Glycinemax)；Mi~ehondrialphosphatetransportprotein；Photoperiod；cDNA-AFLPdoi10．39696．issn．1000—2561．2012．03．010大豆(Gine)作為一種典型的短日照植71()，SOC1等，從而導(dǎo)致莖端分生組織(shoot物．其開花時(shí)間和成熟是農(nóng)業(yè)生產(chǎn)的重要性狀，控apicalmeristem
