花姜酮對(duì)重癥急性胰腺炎大鼠肝損傷的保護(hù)作用.pdf

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1、撒瞳琢學(xué)雜志。2O14，24(2)：9—13⑧2014CHINESEJOURNALOFM1CROC1RCULATIONdoi：10．3969／j．issn．1005～1740．2014．02．003花姜酮對(duì)重癥急性胰腺炎大鼠肝損傷的保護(hù)作用*徐健雄劉黎明鄧文宏趙凱亮左騰何小波郭聞一王衛(wèi)星#[中圖分類號(hào)]R657．51[文獻(xiàn)標(biāo)識(shí)碼]A[文章編號(hào)]1005—1740(2014)02—0009—05目的：探討花姜酮對(duì)重癥急性胰腺炎(SAP)大鼠肝損傷的保護(hù)作用。方法：70只Wistar大鼠隨機(jī)分為4組：正常對(duì)照組(SO組，n一10)；重癥急性胰腺炎組(SAP組，=40)；花姜酮(ZER

2、)預(yù)處理組(ZER組，，2—10)，ZER藥物對(duì)照組(ZER—CON，n一10)。其中sAP組又分為造模1h、3h、6h、12h四個(gè)時(shí)間亞組(均一10)。SAP組及ZER組大鼠采用膽胰管逆行注射5硫磺膽酸鈉(STC)溶液(1ml／100Kg)造模；SO組及ZER—CON組則向膽胰管注入等量生理鹽水。ZER組及ZER—CON組于造模前30min由股靜脈注射10mg／Kg的ZER溶液。比較各組大鼠于造模12h死亡情況、腹水量、血清淀粉酶(AMY)、磷脂酶(PLA)、谷丙轉(zhuǎn)氨酶(ALT)、谷草轉(zhuǎn)氨酶(AST)水平以及胰腺、肝臟組織病理學(xué)評(píng)分(分級(jí))。結(jié)果：ZER—CON組大鼠死亡率、腹

3、水量、AMY、PLA2、ALT、AsT水平以及胰腺和肝臟組織病理學(xué)評(píng)分(分級(jí))與SO組比較，差異均無(wú)統(tǒng)計(jì)學(xué)意義(P>0．05)。SAP組上述指標(biāo)明顯高于SO組，差異有統(tǒng)計(jì)學(xué)意義(均P<0．05)。ZER組上述指標(biāo)與sAP組相比明顯降低(均P<0．05)，但均高于s0組和ZER-c()N組(P

4、Liming，DENGWenhong，ZHAOKailiang，ZUOTeng，HEXiaobo，GUOWengyi，WANGWeixingDepartmentofGeneralSurgery，RenminHospitalofWuhanUniversity，Wuhan430060，China；DepartmentofGeneralSurgery，CentralHospitalofEZhou，EZhou436000，China；Corress-pondingauthor0bjective：Toinvestigatetheprotectiveeffectofzerumboneonse

5、vereacutepancreatitis—associatedliverinjuryinrats．Method：SeventyWistarratswererandomlydividedintofourgroups：shameoperationgroup(SOgroup，一1O)，severeacutepancreatitisgroup(SAPgroup，一40)，zerumbonepretreatmentgroup(ZERgroup，一10)andzerumbonedrugcontrolgroup(ZER-CONgroup，一10)．SAPgroupwasdividedinto

6、fourtimepointsof1h，3h，6hand12h(一10eachtimepoint)．SAPmodelwasinducedbyretrogradeinjectionof5sodiumtaurocholate(O．1ml／100g)inbiliopancreaticductinSAPgroupandZERgroup．RatswereinjectedisotonicsalinesolutioninsteadoftaurocholateasacontrolinSOgroupandZER—CONgroup．10mg／Kgzerumbonesolutionwasadminist

7、eredviafemoralveinhalfhourpriortoestablishingmodelsinZERgroupandZER—CONgroup．AllratsexceptinSAPgroupweresacricedat12htimepointaftertheinductionofsevereacutepancreatitis．TheratsinSAPgroupweresacricedat1h．3h，6hand12h．Theheadofthepancreatictissu