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1、觀賞南瓜病毒病抗性鑒定和抗源材料篩選 摘要:以觀賞南瓜品種迷彩果(CucurbitapepoL.var.kintogaMak.cv.Micaiguo)為研究對象,采用苗期人工接種方法,研究了接種時(shí)期、接種量、培養(yǎng)溫度、接種方法等因素對觀賞南瓜病毒病發(fā)病情況的影響,確定觀賞南瓜病毒病苗期抗性鑒定的最適條件。結(jié)果表明,接種時(shí)期為1片真葉期、接種量為1mg/L、培養(yǎng)溫度為30℃、接種方法為汁液摩擦接種法時(shí)可獲得最高的病情指數(shù),接種效果最佳;采用上述最佳接種條件對11個(gè)觀賞南瓜品種進(jìn)行了苗期病毒病抗性鑒定,篩選出了高
2、抗品種太陽果(C.pepovar.kintogacv.Taiyangguo)、抗病品種紅栗瓜(CucurbitamaximaDuch.cv.Hongligua)和大鈴鐺(C.pepovar.kintogacv.Dalingdang)3份抗源材料。關(guān)鍵詞:觀賞南瓜;病毒?。豢剐澡b定;抗源篩選中圖分類號(hào):S642.1;S432.4+2;S432.2文獻(xiàn)標(biāo)識(shí)碼:A文章編號(hào):0439-8114(2013)12-2836-03ResistanceIdentificationandSelectionofResistantM
3、aterialsAgainstVirusDiseaseofOrnamentalPumpkinFUHong-bing,ZHAOYin-ping(DepartmentofLifeSciences,Xi’anUniversity11ofArtsandScience,Xi’an710065,China)Abstract:TakingornamentalpumpkinvarietyCucurbitapepoL.var.kintogaMak.cv.Micaiguoasresearchmaterial,effectsofin
4、oculationperiod,inoculumdensity,inoculationtemperatureandinoculationmethodsontheincidenceofornamentalpumpkinvirusdiseasewerestudiedbyartificialinoculationatseedlingstagetoobtaintheoptimalconditionsforresistanceidentificationofornamentalpumpkinagainstvirusdis
5、ease.Theresultsshowedthatthehighestdiseaseindexandbestinoculationeffectofvirusdiseasecouldbeobtainedifvaccinationwasatone-leafperiod;inoculumdensitywas1mg/L;inoculationtemperaturewas30℃;andinoculationmethodwasjuicefrictioninoculation.Usingtheinoculationcondi
6、tions,seedlingresistanceidentificationof11ornamentalpumpkinvarietiesagainstvirusdiseasewasconducted.Asaresult,3resistantmaterialsagainstvirusdiseasewerescreenedout,amongwhichC.pepovar.kintogacv.Taiyangguowasidentifiedashighresistance;whileC.maximaDuch.cv.Hon
7、gliguaand11C.pepovar.kintogacv.Dalingdangwereidentifiedasdisease-resistantvarieties.Keywords:ornamentalpumpkin;virusdisease;resistanceidentification;selectionofresistantmaterials觀賞南瓜屬葫蘆科(Cucurbitaceae)南瓜屬(CucurbitaL.)一年生蔓生草本植物,是南瓜屬中一個(gè)特殊類群[1],主要包括3種植物,①中國南瓜(C
8、.moschataDuch.exPoir),又名倭瓜;②印度南瓜(C.maximaDuch.),又名筍瓜;③美洲南瓜(C.pepoL.),又名西葫蘆;以美洲南瓜居多,所以觀賞南瓜的學(xué)名以CucurbitapepoL.var.kintoga11Mak.表述而多見。觀賞南瓜屬于栽培南瓜的變種,是以果實(shí)器官作為觀賞對象的,其果實(shí)大多色彩艷麗,有白、黃、橙、綠等多種顏色,同一果實(shí)甚至還可表現(xiàn)出