苦檻藍葉的化學(xué)成分及其抑菌活性研究

《苦檻藍葉的化學(xué)成分及其抑菌活性研究》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。

1、熱帶亞熱帶植物學(xué)報　2014，22(3)：307~313JournalofTropicalandSubtropicalBotany苦檻藍葉的化學(xué)成分及其抑菌活性研究*葉慧娟,戴航,吳倫秀,郭育暉,谷文祥(華南農(nóng)業(yè)大學(xué)理學(xué)院生物材料研究所，廣州510642)摘要：為研究苦檻藍(MyoporumbontioidesA.Gray)的化學(xué)成分，采用多種柱色譜技術(shù)從苦檻藍葉中分離得到13個化合物，它們的結(jié)構(gòu)分別鑒定為：野黑櫻苷(1)、類葉升麻苷(2)、5,7-二羥基二氫黃酮(3)、3-O-β谷甾醇苷(4)、(3R)-oct-1-en-3-ol-O-β-D-glucopy

2、ranosyl-(1″→2′)-O-β-D-glucopyranoside(5)、7-甲氧基香橙素(6)、異櫻花素(7)、匙葉桉油烯醇(8)、愈創(chuàng)木醇(9)、(1S,2R,5S,6R)-2,6-bis(5-methoxy-3,4-methylenedioxyphenyl)-3,7-dioxabicyclo[3.3.0]octane(10)、(1R,2S,5R,6S)-2-(4-hydroxy-3-methoxyphenyl)-6-(3,4-methylenedioxyphenyl)-3,7-dioxabicyclo[3.3.0]octane(11)、去甲基川陳

3、皮素(12)和3′,4′,5,6,7,8-六甲氧基黃酮(13)，其中化合物4、5、7~11為首次從苦檻藍植物中分離得到。刃天青顯色法測試部分化合物對大腸桿菌與金黃色葡萄球–1菌的抑制活性，結(jié)果表明，5,7-二羥基二氫黃酮(3)對大腸桿菌與金黃色葡萄球菌有一定的抑制作用，其MIC值為62.50μgmL。關(guān)鍵詞：苦檻藍葉；化學(xué)成分；抑菌活性doi:10.3969/j.issn.1005–3395.2014.03.013ChemicalConstituentsfromLeavesofMyoporumbontioidesandTheirBacteriostaticAc

4、tivities*YEHui-juan,DAIHang,WULun-xiu,GUOYu-hui,GUWen-xiang(InstituteofBiomaterial,CollegeofScience,SouthChinaAgriculturalUniversity,Guangzhou510642,China)Abstract:TostudythechemicalconstituentsinMyoporumbontioidesA.Gray,thirteencompoundswereisolatedfromtheleavesofM.bontioidesbyvari

5、ouschromatographytechniques.Onthebasisofspectraldata,theywereidentifiedasprunasin(1),acteoside(2),5,7-dihydroxy?avanone(3),anthemisol(4),(3R)-oct-1-en-3-ol-O-β-D-glucop-yranosyl-(1″→2″)-O-β-D-glucopyranoside(5),7-methoxyaromadenrin(6),5,7-dihydroxy-2-(4-methoxyphenyl)-chroman-4-one(

6、7),spainulenol(8),guaiol(9),(1S,2R,5S,6R)-2,6-bis(5-methoxy-3,4-methylenedioxyphenyl)-3,7-dioxabicyclo[3.3.0]octane(10),(1R,2S,5R,6S)-2-(4-hydroxy-3-methoxyphenyl)-6-(3,4-methylenedioxyphenyl)-3,7-dioxabicyclo[3.3.0]octane(11),demethylnobiletin(12)and2-(3,4-dimethoxyphenyl)-5,6,7,8-

7、tetra-methoxy-4H-chromen-4-one(13).Compounds4,5,and7–11wereobtainedfromM.bontioidesforthe?rsttime.TheinhibitoryactivityagainstStaphyloccocusaureusandEscherichiacoilweredeterminedbytheuseofa96wellplateassay.Theresultsshowedthat5,7-dihydroxy?avone–1(3)possessedmodestlevelofantibacteri

8、alactivitywithMICva