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1、大鼠耳科常規(guī)手術(shù)徑路李鵬1,2丁大連1,2,3高可雷2,3RichardSalvi2,31中山大學(xué)附屬第三醫(yī)院耳鼻咽喉-頭頸外科2CenterforHearingandDeafness,UniversityatBuffalo3中南大學(xué)湘雅醫(yī)院耳鼻咽喉頭頸外科第一作者：李鵬通訊作者：丁大連E-mail:dding@buffalo.edu課題資助：1、NationalInstituteforOccupationalSafetyandHealth：R01OH010235,2、廣東省科技計(jì)劃項(xiàng)目：2011B0318003593
2、、國家外國專家局審批類項(xiàng)目：CG2013440003摘要目的:介紹幾種在大鼠耳科實(shí)驗(yàn)研究中常用的手術(shù)方法。方法:將大鼠麻醉后，根據(jù)不同的實(shí)驗(yàn)需求，分別演示經(jīng)耳后切口的后鼓室徑路、經(jīng)耳上切口的上鼓室徑路、經(jīng)頸部腹側(cè)切口的下鼓室徑路、經(jīng)頸部背側(cè)切口的枕后徑路等手術(shù)方法。結(jié)果：通過不同的手術(shù)徑路可從不同的角度暴露大鼠的中耳和內(nèi)耳，為引導(dǎo)耳蝸生物電反應(yīng)的各種耳蝸電極置放、經(jīng)圓窗膜轉(zhuǎn)染或置藥、藥物經(jīng)耳蝸外淋巴腔灌流、耳蝸內(nèi)淋巴腔灌注、聽骨鏈中斷、以及內(nèi)淋巴囊手術(shù)破壞等各種不同實(shí)驗(yàn)?zāi)康奶峁┳罴咽中g(shù)方案。結(jié)論：根據(jù)大鼠顳骨解剖特點(diǎn)，
3、選擇不同的中耳內(nèi)耳手術(shù)徑路，可以為實(shí)現(xiàn)不同的實(shí)驗(yàn)?zāi)康姆?wù)，對實(shí)驗(yàn)成功提供技術(shù)保障。關(guān)鍵詞:大鼠,中耳,內(nèi)耳,手術(shù)徑路StandardsurgicalapproachesoftheearsurgeryinratsPengLi1,2DalianDing1,2,3KeleiGao2,3RichardSalvi2,31TheThirdAffiliatedHospitalofSunYat-SenUniversity122CenterforHearingandDeafness,UniversityatBuffalo3Xiangya
4、Hospital,CentralSouthUniversitAbstractObject：Tointroduceseveralotologicsurgicalapproachesforspecificexperimentalstudiesinrats.Methods:Basedonthespecificgoalsoftheexperiment,anesthetizedratswerepreparedfortheearsurgeryusingdifferentsurgicalapproachesaimedatoptimi
5、zingtheexperimentaloutcome.Thesesurgicalapproachesincluded:posteriortympanum,superiortympanum,inferiortympanum,andoccipitalsurgeries.Result:Themiddleearcavityandtheinnerearcanbeexposedfromdifferentanglesbyusingdifferentsurgicalapproaches.Forexample,electrodeplac
6、ementforrecordingofcochlearbioelectricresponsescanbeachievedusingapproachesthroughtheposteriortympanumorinferiortympanum.Alternatively,drugdeliveryorgenetransfectionviaroundwindowmembranecanbemosteasilyaccomplishedusingasurgicalapproachfromtheposteriortympanum.C
7、ochlearperfusionofprotectiveorototoxicdrugcanbebestperformedusingtheinferiortympanumapproach.Ossicularchaininterruptiontoinduceaprolongedconductivehearinglosscanbereadilyachievedusingasuperiortympanumapproach.Lastly,surgicaldestructionofendolymphaticsactoinducee
8、xperimentalendolymphatichydropscanbereadilyperformedfromanoccipitalsurgicalapproach.Conclusion:Thesestandardsurgeriescanbeappliedforscientificstudiesoftheearwithdiffe
