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1、中國微侵襲神經(jīng)外科雜志2012年O3月20日第l7卷第3期ChinJMinimInvasiveNeurosttrg，VOL．17，NO．3，March20，2012·107··臨床研究·腦深部電刺激治療帕金森病作用機(jī)制的探討王爾松，季耀東，胡錦，王晨，姚慧斌，呼建文，江澄川【摘要】目的從蛋白組學(xué)的角度探索腦深部電刺激(DBS)與毀損術(shù)治療帕金森病的機(jī)制是否類同。方法采用熒光差異凝膠電泳(DIGE)技術(shù)，測定3例行雙側(cè)丘腦底核DBS治療的帕金森病病人腦脊液中蛋白，在手術(shù)前(未干預(yù)組)、手術(shù)7d后但未刺激前(微毀損組)和刺激1周后(DBS組)
2、的表達(dá)變化。另留取3例非中樞系統(tǒng)疾病病人的腦脊液蛋白作為正常對(duì)照組。結(jié)果未干預(yù)組和微毀損組、未干預(yù)組和DBS組、微毀損組和DBS組的組間比較分別發(fā)現(xiàn)14、18和13個(gè)明顯差異蛋白點(diǎn)。除3個(gè)蛋白點(diǎn)外，未干預(yù)組和微毀損組與未干預(yù)組和DBS組的組間蛋白差異點(diǎn)完全不同；且在這3個(gè)相同的蛋白點(diǎn)中，兩個(gè)蛋白點(diǎn)呈相反方向表達(dá)。結(jié)論結(jié)果初步提示DBS與毀損術(shù)治療帕金森病的機(jī)制不相同?！娟P(guān)鍵詞】帕金森病；腦深部電刺激；毀損術(shù)中圖分類號(hào)：R742．5文獻(xiàn)標(biāo)志碼：A文章編號(hào)：1009—122X(2012)03—01O7—03Studyofmechanismso
3、fdeepbrainstimulationforParkinson’SdiseaseWangErsong，JiYaodong，HuJin，WangChen，YaoHuibin，HuJianwen，JiangChengchuan。1．DepartmentofNeurosurgery，JinshanHospital，F(xiàn)udanUniversity，Shanghai200540，China；2．DepartmentofNeurosurgery，HuashanHospital，F(xiàn)udanUniversity，Shanghai200040，Chin
4、a；3．ResearchCenterforProteomeAnalysis，ShanghaiInstitutesforBiologicalSciences，ChineseAcademyofSciences，Shanghai200031，ChinaAbstract：ObjectiveTostudysimilaritiesanddiferencesinmechanismsbetweendeepbrainstimulation(DBS)andlesioningoperationforParkinson’Sdisease(PD)fromthepe
5、rspectiveofproteomics．MethodsTheproteinsinthecerebrospinalfluidswereassayedbeforeoperation(noninterventiongroup)，the7thdayafteroperationwithnostimulation(slightly-lesionedgroup)，and1weekafterstimulation(DBSgroup)respectively，in3patientswithPDwhohadundergonebilateralsubtha
6、lamicnucleusDBS．Andthecerebrospinalfluidproteinstakenfrom3patientswithnon-centralnervoussystemdiseasesservedasnormalcontrolgroup．Two-dimensionaldiferencegelelectrophoresis(DIGE)techniquewasusedtodeterminetheproteinchange．ResultsTherewere14，18，and13significantlyalteredprot
7、einspotsbetweennoninterventiongroupandslightly-lesionedgroup，noninterventiongroupandDBSgroup，slightly-lesionedgroupandDBSgrouprespectively．Withinthesealteredproteinspotsfromnonintervention／slightly—lesionedgroupsandnonintervention／DBSgroups，therewereonlythreeidenticalprot
8、einspotsinpairedgroups，ofwhichtwowereregulatedindiferentways．ConclusionsOurpreliminaryresultssug
