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1、廣州醫(yī)學(xué)院碩士學(xué)位論文BDE-209對(duì)體外培養(yǎng)海馬神經(jīng)干細(xì)胞增殖分化及凋亡的影響陽(yáng)性,GFAP陽(yáng)性,DAPI陽(yáng)性細(xì)胞數(shù)目,計(jì)算NF+/DAPI+，GFAP+/DAPI+比例，并測(cè)量NF+細(xì)胞突起長(zhǎng)度?！窘Y(jié)果】NF+細(xì)胞比例及NF+細(xì)胞突起長(zhǎng)度隨著B(niǎo)DE-209濃度的增加而減少，GFAP+細(xì)胞比例隨著B(niǎo)DE-209濃度的增加而增加，存在劑量依賴關(guān)系.【結(jié)論】BDE-209可以導(dǎo)致神經(jīng)干細(xì)胞分化成神經(jīng)元的比例發(fā)生改變，隨BDE-209濃度增加神經(jīng)干細(xì)胞分化成神經(jīng)元的比例有不同程度的下降，分化成神經(jīng)元的突起長(zhǎng)度也有不同程度的下降?！娟P(guān)鍵詞】BDE-209;神經(jīng)干細(xì)胞;分化第
2、四部分BDE-209對(duì)體外培養(yǎng)新生鼠海馬神經(jīng)干細(xì)胞凋亡的研究【目的】觀察BDE-209對(duì)海馬神經(jīng)干細(xì)胞凋亡的影響?！静牧吓c方法】1.傳代培養(yǎng)3-4代的新生鼠海馬神經(jīng)干細(xì)胞暴露于BDE-209，實(shí)驗(yàn)共分5組，空白對(duì)照組，DMSO對(duì)照組，實(shí)驗(yàn)A組：BDE-209濃度為10ug/ml,實(shí)驗(yàn)B組：BDE-209濃度為30ug/ml,實(shí)驗(yàn)C組：BDE-209濃度為50ug/ml。每組設(shè)3個(gè)平行樣。DMSO對(duì)照組加入含1‰DMSO的培養(yǎng)液。24h后進(jìn)行檢測(cè)。2.采用免疫細(xì)胞化學(xué)技術(shù)檢測(cè)神經(jīng)干細(xì)胞凋亡?！窘Y(jié)果】不同濃度的BDE-209作用24h后,均可致神經(jīng)干細(xì)胞出現(xiàn)凋亡。實(shí)驗(yàn)A,
3、B,C組作用24h后其凋亡率均有明顯增高，表明BDE-209可導(dǎo)致海馬神經(jīng)干細(xì)胞凋亡,與對(duì)照組相比差異均有顯著意義(P<0.01)?！窘Y(jié)論】BDE-209在一定劑量和作用時(shí)間內(nèi)可致海馬神經(jīng)干細(xì)胞的凋亡。推測(cè)BDE-209在一定濃度范圍內(nèi)可誘導(dǎo)神經(jīng)干細(xì)胞凋亡，這可能是導(dǎo)致仔鼠學(xué)習(xí)記憶能力下降的作用機(jī)制之一?！娟P(guān)鍵詞】BDE-209；凋亡;神經(jīng)干細(xì)胞；6廣州醫(yī)學(xué)院碩士學(xué)位論文BDE-209對(duì)體外培養(yǎng)海馬神經(jīng)干細(xì)胞增殖分化及凋亡的影響RoleofBrominateddiphenlyethers-209inproliferation,differentiationandapo
4、ptosisofneuralstemcellsinVitroPostgraduate:ZhangchunfangTutor:pref.ChendunjinThedepartmentofobstetricsandgynaecologyoftheThirdAffiliatedHospitalofGuangZhouMedicalCollege;TheresearchinstituteofobstetricsandgynaecologyofGuangzhou,ChinaAbstract【Background】1.AboutPolybrominateddiphenlyether
5、s(PBDEs)PBDEsareagroupofbrominatedflameretardant(BFR)chemicalsthatareaddedtomanufacturedproductsincludingpaints,plastics,textiles,andelectronicappliancesandotherfields,endproductswidelyusedforPBDEsincludeelectronics,computers,andfoampaddinginfurniture.Since1970,productionanduseofPBDEsha
6、sresultedinenvironmentalpollutionglobally,Somedatashowedthat80%ofe-wasteindevelopedcountrieswerebrougttoChina,IndiaandPakistanandothercountries,whichalsoaccountedfor90%ofe-wasteinChina.Forexteriorair,urbanPBDEconcentrationswere10timesgreaterthanruralconcentrations,indicatinganurbanrural
7、gradientandgreaterPBDEsourcesinurbanareas,Thereformedcollectionanddistributioncenterfordismantlinge-wasteinsomeareasinChina,PBDEsandotherpersistenttoxicpollutantscontinuouslyreleasetothelocalenvironmentsothattheyresultinseriouspollutionandendangeringhumanhealthbecauseoftheorigi
