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1、DOI:10.16016/j.1000-5404.201409101T細胞因子3在非小細胞肺癌中的表達閔利1,劉煜亮1,徐瑜2,李萬成3,陳虹1(400016重慶,重慶醫(yī)科大學附屬第一醫(yī)院呼吸與危重癥醫(yī)學科1;400037重慶,第三軍醫(yī)大學新橋醫(yī)院呼吸內(nèi)科2;610500成都,成都醫(yī)學院第一附屬醫(yī)院呼吸內(nèi)科3)[摘要]目的研究T細胞因子3(Tcellfactor3,TCF-3)在非小細胞肺癌中的表達及分布情況。方法采用50對人非小細胞肺癌組織標本及其正常癌旁組織對照,進行免疫組化檢測TCF-3蛋白在肺癌組織及正常肺組織中的表達及分布情況;采用5種人非小細
2、胞肺癌細胞系及2種正常人支氣管上皮細胞系進行實時熒光定量PCR,Westernblot檢測肺癌細胞系中TCF-3在mRNA和蛋白水平的表達情況。結(jié)果(1)TCF-3在肺癌組織中表達,表達率為48%(24/50),在正常肺組織中未見表達。TCF-3在肺癌細胞胞核與胞質(zhì)同表達,以細胞核表達為主。(2)在7種細胞系中TCF-3在A549、H520、HBE、BEP-2D4種細胞系中mRNA及蛋白水平均有表達。結(jié)論TCF-3在肺癌組織及細胞系中均表達,TCF-3蛋白在細胞核與細胞質(zhì)同表達,但以細胞核表達為主。[關(guān)鍵詞]T細胞因子3/TCF-3;非小細胞肺癌;Wnt
3、通路[中圖法分類號][文獻標志碼]ATheeExpressionsprofilesofTcellfactor3innon-smallcelllungcancertissuesMinLi1,LiuYuliang1,,XuYu2,LiWancheng3,ChenHong1((1.DepartmentofRespiratoryandCriticalCareMedicine,theFirstAffiliatedHospitalofChongqingMedicalUniversity,Chongqing,400016,China;2.DepartmentofRes
4、piratoryMedicineDiseases,XinqiaoHospital,ThirdMilitaryMedicalUniversity,Chongqing,400037,China;3.DepartmentofRespiratoryDiseases,Medicine,TheFirstAffiliatedHospitalofChengduMedicalCollege,Chengdu,SichuanProvince,610500,China))[AbstractBSTRACT]Objective:Todeterminestudytheexpressi
5、onprofilesanddistributionofTcellfactor-3(TCF-3)innon-smallcelllungcancer(NSCLC).Methods:TCF-3expressionwasexaminedin50NSCLCtissuespecimensnon-smallcelllungcancerspecimensandtheir50correspondingnormallungspecimensbyusingimmunohistochemistrycalassay(S-Pmethod).ThemRNAlevelsofTCF-3i
6、n5NSCLCcelllines(A549,H460,H226,H520,SK-MES-1)and2humannormalbronchiepitheliumcelllines(HBEandBEP-2D)weredetectedin5celllinesofNSCLC(A549,H460,H226,H520andSK-MES-1celllines)and2humannormalbronchialepithelialcelllines(HBEandBEP-2Dcelllines)byreal-timefluorofluorescencegenicquantit
7、ativePCR.TheproteinlevelofTCF-3genewasdetectedbyWesternblotting.Results:(1)Amongthe50lungcancerspecimens,24samplesshowedhighexpressionofTCF-3(48%),includingand22samplesshowedhadtheTCF-3expressionbothinthecytoplasmandnuclei,.ButnoTCF-3expressionwaswerenotdetectedin50correspondingn
8、ormallungspecimens.Amongthe7celllines,.(