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《AFGF、TGFβ1對豬牙乳頭細胞體外增殖分化的實驗研究》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學術(shù)論文-天天文庫。
1、上海交通大學碩士學位論文aFGF、TGFβ1對豬牙乳頭細胞體外增殖分化的實驗研究姓名：余姍姍申請學位級別：碩士專業(yè)：口腔臨床醫(yī)學指導教師：翁雨來20070401上海交通大學２００４級碩士學位論文呈強陽性；細胞在礦化液的培養(yǎng)下可以形成典型的鈣結(jié)節(jié)；并且通過RT-PCR檢測到牙本質(zhì)涎磷蛋白DSPP-mRNA的表達。結(jié)論：豬牙乳頭細胞能夠在體外成功培養(yǎng)，聯(lián)合應用aFGF和TGFβ1不僅可以促進細胞增殖，還能誘導豬牙乳頭細胞向成牙本質(zhì)樣細胞分化，提示我們生長因子誘導后的豬牙乳頭細胞可以作為構(gòu)建牙本質(zhì)牙髓復合體的種子細胞。關(guān)鍵詞：酸性成纖維細胞生長因子，轉(zhuǎn)化生長因子β1
2、，牙乳頭細胞,誘導，分化II上海交通大學２００４級碩士學位論文EXPERIMENTALSTUDYONTHEEFFECTOFaFGFANDTGFβ1ONPROLIFERATIONANDDIFFERENTIATIONOFPORCINEDENTALPAPILLAECELLSINVITROABSTRACTObjective:Thisstudywasdesignedtoculturetheporcinedentalpapillaecells（pDPCs）isolatedfromporcinetoothbudsandtodescribetheirbiologicalcha
3、racteristicsononehand；Ontheotherhand,toinvestigatetheeffectsofacidicfibroblastgrowthfactor（aFGF）andtransforminggrowthfactor-β1（TGFβ1）ontheproliferationanddifferentiationofpDPCs,inthehopeofprovidingtheexperimentalbasisforregenerationofdentin-pulpcomplex.Methods:Dentalpapillaewerecoll
4、ectedfrompostnatalporcinetoothbudatbellstage.Porcinedentalpapillaecellswereisolatedbytheenzyme-digestionmethodcombinedwithmechanicaldissociation,andthenculturedandproliferatedinvitrointhemediumofDMEM/F12containing10%fetalbovineserum.MTTcolorimetricmethodandALPimmunocytochemistrywere
5、usedtoassesstheproliferativeandALPactivityIII上海交通大學２００４級碩士學位論文ofpDPCswhichhadbeentreatedwithaFGFand/orTGFβ1.AcidicFGFandTGFβ1wereaddedintothemediumtoinducethepDPCsdifferentiateintoodontoblast-likecells.Results:Porcinedentalpapillaecellscouldbesuccessfullyculturedinvitroandwereidenti
6、fiedasmesenchymalcells.GrowthfactorswereappliedtoinducethepDPCs.Thefindingssuggestedthatincertainconditions,aFGFcouldsignificantlypromotetheproliferationofpDPCsbutinhibiteALPactivity;TGFβ1couldsharplydecreasetheproliferativeactivitywhileincreaseALPactivityofpDPCs;however,whenthegrow
7、thfactorswereusedincombination,pDPCsshowedremarkablyproliferationaswellasdifferentiationabilitysuchassingle-polarcytoplasmicprocess,stronglypositiveALPstaining,classicalcalciumnoduleformationandmRNAexpressionofdentinsialophosphoprotein（DSPP）.Conclusions:Theporcinedentalpapillaecells
8、canbesuccessfullycu
