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《金黃色葡萄球菌β溶血素基因的克隆與表達》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、中文摘要金黃色葡萄球菌B．溶血素是金黃色葡萄球菌分泌的外毒索之一。是重要的毒力因子，它可引起多種疾病，奶牛乳房炎的主要致病因子之一就是金黃色葡萄球菌分泌的B．溶血素。奶牛乳房炎是一種多因素疾病，給世界各國奶牛業(yè)造成巨大的經(jīng)濟損失?；寂ＥＤ讨畜w細胞數(shù)大幅增加，會降低乳蛋AbstractBeta-hemoIysinwaSoneofthemostimportantextracellulartoxinsproducedbyviruientStrainsof研qp妙幻∞cc淞d“陽琊(s口“理時)，whichwaSanimpon
2、antvirulencef犯torandassociatedwjththeeti0109icalofavarjetyofjnfectiousdiseasesjnanimaIs，includingmastjtisMaStitiswaSakjndofmu】tiplef如tOrdisease，whichresultedinenomouseconomiclossinwo—darIdplayedanimpon鋤tro】einthemilkquality．ThesomaticnumbcrofthemiIkofthemastitis
3、ca砌ewasincreaSed，whichcoulddecreasethecontentionofmjlkprotein，1actoseandmilkfat吼Ifantibioticswereusedtotreattheinfectedcanle，whichmight1eadtomuch『emajnDoublep01lutionofthesomaticandantibioticjnfreshmilk，mightbringd枷agetothemilkproductintheaspectofqual時鋤dnavoLMas
4、titiswaSap—maryobstacletomedeVelopmentofa11imajhusballdryofChina，foran廿biotictreatlnentSaffbctedthemilkqualjtyseriously'thusvaccinationmightbeawaytopreventt}1edisease．Asaresult，todevelop廿1eengineeringVaccinemightbeawaytosolVe也eproblem．As仃ainof最4“旭淞SlOusedi11this
5、researchwasgained行om廿1emastitismilks鋤ples．Thegeneencodingbeta．hemolysinof腳砂，DcDcc塒口舯塒was啪plifiedbyPolymerasechainReaction(PcR)鋤dwaslinkedtot11eph缸)-18一TtoconsⅡucttbeB-hemolysincIoneplaSmid．ThecompleteDNAsequenceofthecloned8-hemolysingene療Dm＆矽砂kDcc蚶日“旭淞wassequenc
6、酣aI】dconfi姍edbyDNAsequencea11alysis．Theresultsshowed廿1att}leDNAsequencehom0109ywaSmoret11an99．2％aftercomparingw油sequencepublishedinGenbank，鋤dt11ehomologyoftheencodingproteinwas100％，A11tllisassuredthatthesequenceoftlle且-hemolysinof飄礎(chǔ)yfococc淞口“雕淞hadhi曲conserv撕on鋤d
7、homolog弘Thent}IerecombinationwaSdoubleen巧meinciteda11dinsertedimopET-32-aexpressionvector．TheexpressionofrecombinarItbeta-hemolysinproteinwasdetcctedbySDS母AGEaIldWestemBlot．Theresultsshowed出atthemolecuIarweightoffusionproteinofbeta小em01ysinwasabout57l(Da，鋤dexpre
8、ssionlevelw鵲30％．TheexpressionofrecombinalltproteinwaSpurined，then也eproductwasusedin血etreadmjlltest，thereSultsshowedt11att11eexpressionpmteinalsocou】dmakethesheeperyth
