CO2對人卵巢癌SKOV3細胞裸鼠皮下移植瘤生長和轉(zhuǎn)移的影響

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1、CO2對人卵巢癌SKOV3細胞裸鼠皮下移植瘤生長和轉(zhuǎn)移的影響作者：王亞平,張愛榮單位：山東大學(xué)第二醫(yī)院婦產(chǎn)科，濟南【摘要】目的探討不同壓力持續(xù)性CO2氣腹對人卵巢癌SKOV3細胞裸鼠皮下移植瘤生長和轉(zhuǎn)移的影響。方法將體外培養(yǎng)的人卵巢癌細胞株SKOV3分別暴露于不同壓力（8、10、12mmHg）和作用時間（1、3h）的CO2環(huán)境中，對照組細胞放入CO2培養(yǎng)箱常規(guī)培養(yǎng)。收集各組細胞后接種于裸鼠腋下，建立有差異的裸鼠皮下移植瘤模型并繪制腫瘤生長曲線。接種6周后脫臼處死全部裸鼠，測量移植瘤的體積和重量，采用免疫組織化學(xué)法檢測各組中增殖細胞核抗原（PCN

2、A）和nm23-H1蛋白的表達。結(jié)果接種7d后各組均明顯成瘤，各CO2氣體處理組的皮下移植瘤生長速度均快于對照組，其皮下移植瘤體積和重量也高于對照組（P＜0.01）。各CO2處理組中腫瘤細胞PCNA蛋白表達陽性率均高于對照組（P＜0.01），各組間nm23-H1蛋白表達陽性率差異無統(tǒng)計學(xué)意義(P＞0.05)。結(jié)論CO2氣體本身對人卵巢癌SKOV3細胞裸鼠皮下移植瘤的生長有明顯的促進作用，對轉(zhuǎn)移影響不大?！娟P(guān)鍵詞】卵巢腫瘤；二氧化碳；小鼠，裸；移植瘤　　Effectofcarbondioxideonthegrowthandmetastasisof

3、thehumanovariancarcinomacelllineSKOV3xeno-transplantedintonudemice　　WANGYa-ping,ZHANGAi-rong　?。―epartmentofObstetricsandGynecology,SecondHospitalofShandongUniversity,Jinan250033,China）　　Abstract:ObjectiveToevaluatetheeffectsofCO2pneumoperitoneumonthegrowthandmetastasisoftheh

4、umanovariancarcinomacelllineSKOV3inanimalmodels.MethodsFirst,theSKOV3ovariancancercellswereexposedtoCO2-insufflationatdifferentcarbondioxidepressures(8mmHg,10mmHgand12mmHg)andeffecttimes(1h,3h).Then,eachmousewasinoculatedwith0.2mLcellsuspension(including6.0×106tumorcel

5、ls).Thegrowthstatusofthetumorwasregularlyobserved,andthegrowthcurvewasrecorded.6weekslater,allmicewereexecutedbyluxation.Themicewereimmediatelyanatomizedandthevolumeandweightoftumorsweredetermined.ImmunohistochemistrywasusedtodeterminetheexpressionofPCNAproteinandnm23-H1prot

6、einofthesecells.Results7daysafterhypodermicinoculation,tumorgrowthoccurredinallthegroups.Thetumorgrowthwasreinforcedintheexperimentalgroupscomparedwiththoseofthecontrolgroup(P＜0.01).TheexpressionofPCNAproteinwashigherinexperimentalgroupsthanthatinthecontrolgroup(P＜0.01).Butt

7、herewasnosignificantdifferencebetweenthegroupsintheexpressionofnm23-H1protein(P＞0.05).ConclusionCarbondioxidehasauxoactiononthegrowthofthehumanovariancarcinomacelllineSKOV3xeno-transplantedintonudemice,buthasnoeffectsontumormetastasis.　　Keywords:Ovarianneoplasma；Carbondioxid

8、e；Mice，nude；Xenografttumor　　腹腔鏡手術(shù)在婦科良性腫瘤中的應(yīng)用已得到肯定，但腹腔鏡能否應(yīng)用于婦科惡性腫瘤的手術(shù)一直存有爭議。