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1、萬方數(shù)據(jù)中國組織工程研究與臨床康復(fù)多事73眷多亨27勇鏟2009—07—02出版JoumalofClinicalRehabilitativeTissueEngineeringResearchJury2，2009VoL13,No．27j災(zāi)瓦震羲強(qiáng)匿掌骨髓間充質(zhì)干細(xì)胞體外誘導(dǎo)培養(yǎng)后的電生理特性☆黨懿，齊曉勇，孟存良，李樹仁，李英肖，劉惠良，王天紅Electrophysiologicalcharacteristicsofbonemaffowstemcellsfollowing／nv／troinductionDangY1，QiX
2、iao-yong．MengCun-liang，UShu怕n，UYing-xiao。UuHuHiang。Wang"rian-hongBACKGROUND：BonemarrowmesenchymaIstenlcells(BMSCs)cangrowinhostmyocardium，differentiateundermyocardialcondition，improvecardiacfunction．However，biologicaIcharacteristicsofBMSCdifferentiationarestilIun
3、dearpresently．OBJECTIVE：TOstudytheexpressionandelectrophysiologicalcharacteristicsofBMSCinvitroconnexin-43following5-azacitidine(5-aza)treatment．DESIGN。TIMEANDSETTING：111ecytologicalinvitrocontrolledstudywasperformedattheHeartCenter。HebeiProvindaIPeople’sHospitaI
4、的mJuly2007toFebruary2009．MATERIALS：Atotalof24malepigsaged2monthswerepurchasedfromExpecimentalAnimalCenter,Heb萌MedicaIUniversity．METHODS：BilateralfemoralbonemarrowwasobtainedfrOmpigsundersterilecondition．BMSCswereharvestedbyPercolIdensitygradientinv#ro．Atpassage2。
5、BMSCsweretreatedwith10pmol／L5．a(chǎn)za．a(chǎn)ndincubatedinDMeMwithoutinductor24hoursIater．1ndicesweremeasured1。2。3weeksfollowinginduction．A∞ntroIgroupwassetup，whichwasnottreatedwith5．a(chǎn)zacitidine．Followingbonemarrowextraction．experimentalpigswereanesthetizedtoobtainventricu
6、larmusde．NormaIventricularmusdecellswerejsolatedandculturedbytissueblockenzymedigestionmathod．MAlN0UTCoMEMEASURES：Expressionofconnexin-43wasmeasuredbyImmunohistochomicaIstaining(ASCmethod)．Itocurrentdensityandactionpotentialweredaterminedbypatchdamptechnique．RESU
7、LTS：At1and2weeksfoIlowing5-azainduction．someBMSCswereposiUveforconnexin一43，withthepresenceofbrownpartidsssurroundingnudei．At3weeks．positiverateofconnexin-43was95％．TheareawithlargecelIdensitywaspresented枷thsimilarstructuretonorrnaImyocardium．At+80mV．comparedwithno
8、rmaImyocardialcells，ItocurrentdensitywassignificantlyreducedinBMSCsfollowing1and2weeksandinthecontrolgroup(P‘0．05)．Itocurrentdensitywassignificantlyincreasedto
