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1、中西醫(yī)結(jié)合學(xué)報(bào)2004年9月第2卷第5期JChinIntegrMed,Sep2004,Vol.2,No.5·375·大鼠骨髓間充質(zhì)干細(xì)胞體外誘導(dǎo)條件下的成骨特性111213456陳東風(fēng),黎暉,周健洪,謝瑤,李伊為,杜少輝,張宜,黃潔,徐敏(1.廣州中醫(yī)藥大學(xué)解剖學(xué)教研室,廣東廣州510405;2.中山大學(xué)中山醫(yī)學(xué)院解剖學(xué)教研室,廣東廣州510080;3.深圳市中醫(yī)院內(nèi)科,廣東深圳518000;4.深圳市沙河醫(yī)院,廣東深圳518000;5.深圳市人民醫(yī)院,廣東深圳518000;6.香港浸會大學(xué)中醫(yī)藥學(xué)院)[摘要]目的:探討大鼠骨髓間充質(zhì)干細(xì)胞(mesenchymal
2、stemcells,MSCs)在誘導(dǎo)條件下的成骨特性。方法:使用密度梯度法分離成年大鼠骨髓MSCs,以地塞米松、β-甘油磷酸鈉、維生素C為成骨誘導(dǎo)劑。結(jié)果:形態(tài)學(xué)表明,MSCs貼壁細(xì)胞呈集落生長,有成纖維細(xì)胞外觀。地塞米松低劑量組誘導(dǎo)7d后堿性磷酸酶(alkalinephosphatase,ALP)表達(dá)明顯升高,陽性細(xì)胞數(shù)為(15.1±2.6)個(gè),而對照組ALP表達(dá)較弱,陽性細(xì)胞數(shù)為(12.0±3.5)個(gè),兩組比較P<0.01。地塞米松成骨誘導(dǎo)劑低劑量組促進(jìn)MSC成骨,在誘導(dǎo)7d時(shí)即出現(xiàn)鈣化結(jié)節(jié),而對照組未出現(xiàn);地塞米松低劑量組誘導(dǎo)21d鈣化結(jié)節(jié)為(9.0±1.7
3、)個(gè),而對照組為(2.0±1.8)個(gè),兩組比較P<0.01。結(jié)論:低劑量地塞米松成骨誘導(dǎo)劑能誘導(dǎo)MSC向成骨細(xì)胞分化,可以為體內(nèi)骨組織工程提供種子細(xì)胞。[關(guān)鍵詞]干細(xì)胞,骨髓;成骨細(xì)胞;動(dòng)物,實(shí)驗(yàn)[中圖分類號]R329.471[文獻(xiàn)標(biāo)識碼]A[文章編號]1672-1977(2004)05-0375-04Osteogenesischaracteristicsofculturedratmesenchymalstemcellsunderboneinductioncondition111213456CHENDong-Feng,LIHui,ZHOUJian-Hong,XIE
4、Yao,LIYi-Wei,DUShao-Hui,ZHANGYi,HUANGJie,XUMin(1.DepartmentofAnatomy,GuangzhouUniversityofTraditionalChineseMedicine,Guangzhou,GuangdongProvince510405,China;2.DepartmentofAnatomy,ZhongshanMedicalCollege,SUNYat-senUniversity,Guangzhou,GuangdongProvince510080,China;3.DepartmentofInterna
5、lMedicine,ShenzhenHospitalofTraditionalChineseMedicine,Shenzhen,GuangdongProvince518000,China;4.ShenzhenShaheHospital,Shenzhen,GuangdongProvince518000,China;5.Shenz-henPeople’sHospital,Shenzhen,GuangdongProvince518000,China;6.SchoolofTraditionalChineseMedicine,HongKongBaptiseUnivesity
6、,HongKong,China)ABSTRACTObjective:Toinvestigatetheosteogenesischaracteristicsofculturedratmesenchymalstemcells(MSCs)underboneinductioncondition.Methods:MSCswereisolatedfromadultratbyusingdensitygradientseparationmethod.Theosteo-genicinducerswerecompoundsofDexone,β-glycerophosphatesodi
7、umandvitaminC.Results:TheMSCattachmentformedsoonaftertheseedingandgrewintocolonieswiththeappearanceoffibroblasticcells.TheosteogenicinducerwithlowdoseofDexonecouldpromotetheosteogenicdifferentiationofMSC.InthegroupofosteogenicinducerwithlowdoseofDexone,theexpressionofalkalinephosphata
8、se(AL
