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1、中國組織工程研究與臨床康復(fù)第11卷第33期2007-08-19出版JournalofClinicalRehabilitativeTissueEngineeringResearchAugust19,2007Vol.11,No.33CRTER基礎(chǔ)醫(yī)學(xué)人脂肪干細(xì)胞體外培養(yǎng)特性及成脂成軟骨分化*☆郭寶鋒,尹維田,劉浩宇,魏壯DepartmentofHandInvitrocultureanddifferentiationofhumanadipose-derivedmesenchymalstemcellsinto
2、Surgery,China-JapanadipocytesandchondrocytesUnionHospitalofJilinUniversity,ChangchunAbstract130033,JilinProvince,ChinaAIM:Toinvestigatethebiologicalfeaturesofhumanadipose-derivedmesenchymalstemcells(ADMSCs)toisolateandculture,thendifferentiateintoadipoc
3、yteandchondrocyteinvitro,anddiscussthefeasibilityofADMSCsasseedcellsinGuoBao-feng☆,cartilagetissueengineering.Studyingfordoctorate,DepartmentofHandMETHODS:TheexperimentwasconductedintheLaboratoryofProstateDiseasesPreventionandTreatmentResearchSurgery,Ch
4、ina-JapanCenter,China-JapanUnionHospitalofJilinUniversityfromAugust2006toMarch2007.①HumanadiposewasobtainedUnionHospitalofJilinUniversity,ChangchunfromthehealthywomanhoodduringtheabdominalliposuctionprocedureanddigestedwithcollagenaseⅠtoisolate130033,Ji
5、linProvince,ADMSCs,whichwereincubatedinhigh-glucoseDMEMwith0.10volumefractionfetalbovineserum(FBS)forprimaryChinagbf_cc@sohu.comculture.②Growthcurveofthethird-passageADMSCswasdrawnbycytometry;theexpressionsofCD13,andCD34wereassayedbyflowcytometryandimmu
6、nofluorescencetechnique.③Thethird-passageADMSCswereculturedinCorrespondenceto:high-glucoseDMEMsupplementedwith0.10volumefractionFBS,0.5mmol/LIBMX,1μmol/LdexamethasoneandWeiZhuang,Attendingphysician,10μmol/Linsulinforadipogenicinduction,andwith0.01volume
7、fractionFBS,10μg/Ltransforminggrowthfactor-β1DepartmentofHand(TGF-β1),50nmol/Lascorbate-2-phosphateand6.25mg/Linsulinforchondrogenicinduction.④TheappearanceandSurgery,China-JapanUnionHospitalofJilingenerationofADMSCswereobservedeverydayunderinvertedmicr
8、oscope,andthedifferentiationstoadipocyteandUniversity,ChangchunchondrocytewereverifiedbyoilredOstaining,AB-PSAstainingandimmunocytochemicalstaining.130033,JilinProvince,RESULTS:①ADMSCsculturedinvitrowereinflatfusiformshapeandstab
