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1、VeterinaryMicrobiology,31(1992)11-1811ElsevierSciencePublishersB.V.,AmsterdamExperimentalinfectionofpigswiththeporcinerespiratorycoronavirus(PRCV)"measureofviralexcretionE.Bourgueil,E.Hutet,R.CarioletandP.VannierMinist~redel'AgricultureetdelaFor~t,CNEV
2、ALaboratoiredeRecherchesAvicoleetPorcine,StationdePathologiePorcine,BP53,22440Ploufragan,France(Accepted30August1991)ABSTRACTBourgueil,E.,Hutet,E.,Cariolet,R.andVannier,P.,1992.Experimentalinfectionofpigswiththeporcinerespiratorycoronavirus(PRCV):measu
3、reofviralexcretion.Vet.Microbiol.,31:11-18.Twelvepigswereexperimentallyinfectedwithaporcinerespiratorycoronavirus(PRCV)bytheoronasalroute.Viralexcretionwasmeasureddailybytwomeans-deepnasalswabsandairsamplesobtainedinacyclonesampler.Clinicalsignswerever
4、yslightoninfectedpigs.Airborneviruscouldberecoveredfromday1today6post-infectioninthecyclonesampleraswellasinpetridishesplacedinthesameloose-box.Viraltitresobtainedfromnasalswabsweresignificantlycorrelatedwiththoseobtainedfromairsamples.Differentcollect
5、ionmediawerecompared.Themostefficientmediaforthecollectionofinfectiousviralparticlescontainedaprotectiveagentsuchasfoetalcalfserum.INTRODUCTIONRespiratorydisordersduetovirusesarethemainproblemsrecordedinareasofintensiveporcineproduction.Commoncausesoft
6、hediseasein-cludespseudorabiesvirus(PRV),influenzavirusesandtheporcinerespira-torycoronavirus(PRCV),thelatterbeingfirstdetectedindifferentEuro-peancountriesin1983-1984(Brownetal.,1986;Duretetal.,1988;Pensaertetal.,1986).Serologicalstudieshavedemonstrat
7、edacloserelationshipbe-tweenPRCVandtransmissiblegastroenteritisvirus(TGEV)withthevi-rusesbeingdistinguishedfromeachotheronlybytheuseofmonoclonalan-tibodies(Laudeetal.,1988;Garwesetal.,1988).Recently,Rasschaertetal.(1990)comparedthegenomicorganizationof
8、eachvirusandshowedthatPRCVhadvariousnucleotidedeletionswhencomparedtoTGEV.Nocon-clusioncouldbemadeconcerningthegenomicmodification(s)controllingthephenotypicdifferenceobservedbetweentheviruses,namelytheimpairedmultiplicationofPRCVinthed
