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1、ResearchinVeterinaryScience/989,47,23-29Pathogenicityofexperimentalinfectionwith'pneumotropic'porcinecoronavirusD.O'TOOLE,I.BROWN,A.BRIDGES,S.F.CARTWRIGHT,MinistryofAgriculture,FisheriesandFood,CentralVeterinaryLaboratory,NewHaw,Weybridge,SurreyKT153N
2、BViruslocalisationandlesionswerestudiedin14one?tionalbreedswereused.Theywerehysterotomyweek-oldpigletsfollowingcombinedintranasal-oralderivedoneortwodaysbeforetheirduedateandsub?inoculationwithaBritishisolateof'pneumotropic'sequentlycolostrum-deprived
3、usingamodificationofporcinecoronavirus(pcv)andwerecomparedwiththemethodsdescribedbyTavernoretal(1971).Thetheeffectsoftransmissiblegastroenteritisvirusthreeparentsowswereseronegativetor-evITGEVand(TGEV)infectioninfivepiglets.UnlikeTGEV-infectedoriginat
4、edinherdswithnoprevioushistoryoftrans?piglets,allrcv-moculatedpigletsremainedclinicallymissiblegastroenteritis.Pigletswererearedindi?healthy.Seroconversionwasdetectedatsevendaysviduallyinsterilealuminiumanodisedcagesinplasticafterinoculation.Mildbronc
5、hointerstitialpneu?isolatorskeptat20to25°Cwithpositivepressuremoniainvolvingterminalairwayswasconsistentlyventilation.Theywerefedonadietofsterilisedpresentattwodaysafterinfectionandthereafter.evaporatedmilk(CarnationFoods)dilutedwithaBothPCVandTGEVinf
6、ectedbronchiolarepitheliummineralsolutioncontainingferroussulphatethree,andalveolarmacrophagesbut,unlikeTGEV,replica?fourorfivetimesdailyaccordingtoaschedulebasedtionbyrevinvillousenterocyteswaslimitedanddidonage.Pigletswerealsogivenanoralvitaminnotca
7、usevillousatrophy.supplement(AdibecDrops;Parke,Davis).ANEWcoronavirusofpigswhichhasacloseanti?genicrelationshiptotransmissiblegastroenteritisExperimentaldesignvirus(TGEV)hasrecentlybeenidentifiedasenz0'1ticinAsonly12singleisolatorswithindividualcagesG
8、reatBritain,France,BelgiumandHoIlandwereavailable,thestudywasdoneintwoperiodsone(reviewedbyJestinetal1987).Conventionalsero?monthapart.Thetwobatchesofpigletsweremain?logicaltestsdonotdistinguishbetween'pneumo?tainedunderidenticalconditions.The
