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1、提取桐與應(yīng)用食品科技FOODSCIENCEANDTECHNoLOGY2014.~第3蜷第蝴超微粉碎與微波輔助酶法提取玉米黃色素工藝優(yōu)化李曉玲一,陳相艷,王文亮,徐同成,弓志青,王世清(1.青島農(nóng)業(yè)大學(xué)食品科學(xué)與工程學(xué)院,青島266109;2.山東省農(nóng)業(yè)科學(xué)院農(nóng)產(chǎn)品研究所,濟(jì)南250100)摘要:采用超微粉碎技術(shù)處理原料玉米蛋白粉,在Plackett—Burman設(shè)計(jì)、最陡爬坡試驗(yàn)的基礎(chǔ)上選取加酶量、微粉粒度、微波功率為自變量,并以玉米黃色素的提取率為響應(yīng)值進(jìn)行響應(yīng)面優(yōu)化設(shè)計(jì)。研究結(jié)果表明:玉米黃色素最大吸收峰為447nm,最陡爬坡試驗(yàn)表明響應(yīng)中心為:加酶量l%、微粉粒度300目、微
2、波功率300W,最后利用Box—Behnken響應(yīng)面分析建立了3#-關(guān)鍵影響因素的三元二次多項(xiàng)式數(shù)學(xué)模型:Y=0.57+0.026X】+0.046X2-0.015X3+8.25×10~X】X2—8.00X10-XX+5.00×10~X,X3-2.7l×10X12+829×10X22+9..04X10X,得到最佳提取工藝為:加酶量1.25%,微粉粒度400目,微波功率200W。模型預(yù)測(cè)結(jié)果提取率為0.688mg/g,驗(yàn)證試驗(yàn)結(jié)果提取率達(dá)0.672mg/g,與預(yù)測(cè)值吻合相對(duì)良好。關(guān)鍵詞:超微粉碎;微波酶法;玉米黃色素;響應(yīng)面中圖分類號(hào):TS264.4文獻(xiàn)標(biāo)志碼:A文章編號(hào):1005—9
3、989(2014)02—0228—06Optimizationofultrafinecomminution-assistedextractionofmaizeyellowpigmentbymicrowavetreatmentandenzymehydrolysisLIXiao—ling,CHENXiang—yan,WANGWen—liang,XUTong—cheng,GONGZhi—qing.WANGShi—qing(1.FoodScienceandEngineeringCollege,QingdaoAgriculturalUniversity,Qingdao266109;2.Ins
4、tituteofAgro-FoodScienceandTechnology,ShandongAcademyofAgriculturalScience,Jinan250100)Abstract:Rawmateria1wastreatedbyUltrafinecomminution.BasedonPlackett.Burmandesignandsteepestascentexperiment.enzymeaddition。particlesizeandmicrowavepowerwereselectedasjndependentvariables.Theextractionratewa
5、salsosolectedasresponsevaluetoconducttheoptimizationofresponsesurfacedesign.Resultsshowedthatthemaximumabsorptionpeakwason447nm.ResultsofthesteepestascentexperimentindicatedthattheresponsecenterwasasfoIlows:enzymeadditionof1%.particlesizeof300mesh,andmicrowavepowerof300W.WithBoxBehnkenresponse
6、surfaceanalysis,a3一factorand2.degreemathematicaImode1wasestablishedasfoIlows:Y:0.57+0.026X。+0.046X,一O.015X3+8.25×10一X1X2-8.00×10一X1X3+5.OO×10‘X?X3-2.71×10一X12+829×1O’X22+9..04×1O。X3.The收稿19期:2013—08—05通訊作者基金項(xiàng)目:國(guó)家自然科學(xué)基金項(xiàng)目(31271963);山東省農(nóng)業(yè)重大應(yīng)用技術(shù)創(chuàng)新課題(魯財(cái)農(nóng)指[2O11139)。作者簡(jiǎn)介:李曉玲(1988-),女,山東安丘人,碩士研究生,研究方
7、向?yàn)槭称钒踩2亍!?28‘食品科技2014年第3蜷第0蛹FOODSCIENCEANDTECHNOLOGY提取掏與應(yīng)用opltimumextractionconditionswereasfoIlows:enzymeadditionof1.25%。particlesizeof400mesh。andmicrowavepowerof200W.Basedonthemodel,theextractionratedwasexpectedtoreach0。688mg/g.Resul