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1、安徽農(nóng)業(yè)大學(xué)學(xué)報，2012，39(5)：677．681JournalofAnhuiAgriculturalUniversity網(wǎng)絡(luò)出版時間：2012．8．2712：04：03[URL】http：／／www．cnki．net／kcms／detail／34．1162．S．20120827．1204．028．html大豆疫病拮抗細菌的篩選及生防作用楊光紅，高智謀，曹舜，江濤，汪濤(1．安徽農(nóng)業(yè)大學(xué)植物保護學(xué)院，合肥230036；2安徽農(nóng)業(yè)科學(xué)研究院植物保護研究所，合肥230031)摘要：從大豆根圍土樣中篩選得到對大豆疫霉有較好拮抗作用的生防菌株4株，經(jīng)平板測定，生防菌株BY-
2、2的防效最好，達79．o0％；對其不同發(fā)酵時間的代謝液活性進行測定，發(fā)現(xiàn)72h代謝液的抑制效果最佳，達到40．93％BY-2代謝液能顯著抑制大豆疫霉游動孢子萌發(fā)，抑制效果為39．89％～57．71％；其揮發(fā)性物質(zhì)能顯著抑制疫霉菌絲的生長，抑制效果為81．70％～91．94％。離體和盆栽試驗結(jié)果表明，BY-2菌液處理對大豆疫病具較好的防治效果，離體葉片防效和盆栽防效分別為63_38％和75．01％。此外，用BY-2活菌液灌根大豆幼苗的鮮重、干重、根長和莖長顯著增加，表明生防菌株BY-2能促進大豆植株的生長。關(guān)鍵詞：生防細菌；大豆疫霉；生防效果；促生作用中圖分類號：$435
3、．651文獻標(biāo)識碼：A文章編號：1672—352X(2012)05—0677—05ScreeningandbiocontroleffectsofantagonisticbacteriaagainstPhytophthorasojaeYANGGuang—hong，GAOZhi．moulCAOShunlJIANGTao，WANGTao2，，(1．SchoolofPlantProtection，AnhuiAgriculturalUniversity,Hefei230036；2．InstituteofPlantProtection，AnhuiAcademyofAgricultu
4、ralSciences，Hefei230031)Abstract：Fourbacterialstrainswereisolatedfromthesoilinrhizosphereofsoybcanplants．AntagonistictestsshowedthatBY-2stronglyrestrictedthegrowthof1，tophthorasojae．Inthedual—culturetestinplates，theresultsshowedthatthebacterialcultureofstrainBY-2significantlyinhibitedthe
5、hyphalgrowthofPsojae,withahighinhibitionrateof79．0O％．TheresultsalsoshowedthattheinhibitionactivityoffermentationliguidagainstBY_2variedwithtime．a(chǎn)ndtheinhibitionofmetabolicliquidat72htreatmentagainstPsojaewas40．93％，beingthehighest．Themetabolicliquidcouldevidentlyinhibitgerminationofzoospo
6、res，withtheinhibitoryratesfrom39．89％to57．71％．ThevolatilesfromBY-2couldevidentlyinhibithyphalgrowthofPsojae，withtheinhibitoryratesfrom81．70％tO91．94％．Detached1eavesandpotexperimentsdemonstratedthatthebacteria1ofBY-2hadsignificantcontrolonsoybeanblight．withthecontroleffectivenessbeing63．38％
7、and75．01％，respectively．Inaddition，thefleshweightanddryweightwereallincreasedsignificantlyandthegrowthofsoybeanwaspromotedafterrootaffusedwithBY2bacterialsuspension．Keywords：antagonisticbacteria；Phytophthorasojae；biologicalcontrol；growthpromoting由大豆疫霉(PtophthorasojaeKaufma
