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1、熱帶作物學(xué)報2014,35(5):992—998ChineseJournalofTmpica1Cro千層金精油有機溶劑提取工藝的優(yōu)化及成分分析葉征美,劉鑫,-,沈明娟,一,陳建煙1,2陳曉丹.一,李永裕,2聿,吳少華,z1福建農(nóng)林大學(xué)園藝學(xué)院.福建福州3500022福建農(nóng)林大學(xué)園藝植物天然產(chǎn)物研究所.福建福州350002摘要采用有機溶劑萃取法提取千層金精油,在單因素試驗的基礎(chǔ)上,以石油醚(60~90)為提取溶劑.選取提取時間、提取溫度、料液比為影響因素,用正交試驗優(yōu)化千層金精油的提取工藝.并采用氣相色譜一質(zhì)譜(GC—MS)技術(shù)鑒定了千層金
2、精油的化學(xué)成分。結(jié)果表明,千層金精油的最佳提取工藝條件為:提取時間3h.提取溫度50℃,料液比1:20,在此工藝條件下的精油提取得率4.51%。GC—MS分析結(jié)果表明.千層金精油共含有41個組分,主要成分為甲基丁香酚,相對含量達到了83.55%,其次為肉桂酸甲酯(4.55%)、3,4.5一三甲氧基苯甲酸甲酯(1.8l%)、大根香葉烯D(1_36%)。關(guān)鍵詞千層金;精油;有機溶劑提取;正交試驗;氣相色譜一質(zhì)譜中圖分類號TQ65文獻標(biāo)識碼AOptimizationofProcessforExtractionfromMelaleucabract
3、eataEssentialOilwithOrganicSolventandChemicalCompositionIdentifyYEZhengmeiLIUXin,SHENMingjuan,CHENJianyan'2CHENXiaodan.LIYongyu.WUShaohua,21CollegeofHorticulture,F(xiàn)~jianAgriculturalandForestryUniversity,Fuzhou,凡m350002,China2InstituteofHorticulturalPlantsNaturalProducts,F(xiàn)u
4、jianAgricultureandForestryUniversity,Fuzhou,阿350002,ChinaAbstractTooptimizetheextractionprocessofMelaleucabracteataessentialoilbyorganicsolvenLUsingpetroleumether(boilingpoint60-90℃)astheextractingsolvent.Theefectsofextractiontime,extractiontemperatureandmaterial—liquidra
5、tioontheextractionbracteataoilwereselectedfororthogonaldesigntooptimizetheextractionprocessbasedonsinglefactortests.Gaschromatography-massspectrometry(GC—MS)technicalwasusedtoidentifythechemicalcompositionof艙bracteataessentialoil.Theresultsindicatedthattheoptimumextractio
6、nconditionwasthat:extractiontime3h,extractiontemperature50cC,material-liquidratio1:20.ThechemicalcomponentswereanalyzedbyGC—MS,and41peakswereseparatedandidentified.Thetestresultsshowedthatthemaincomponentoftheessentialoilofbracteataleaveswasmethyleugeno1.a(chǎn)nditsrelativecon
7、tentwas83.55%,followedbymethylcinnamate(4.55%),benzoicacid,3,4,5一trimethoxy一,methylester(1.81%),andgermacreneD(1_36%).KeywordsMelaleucabracteata;Essentialoil;Extractionbyorganicsolvent;Orthogonaldesign;GC—MSdoi10.3969/j.issn.1000—2561.2014.05.027千層金(Melaleucabracteata)。又名
8、黃金寶樹、世界上最流行、視覺效果最好的色葉喬木新樹種之黃金香柳,屬于桃金娘科白干層屬.為常綠喬木。一,可用于庭園景觀、道路美化、社區(qū)綠化,也可原產(chǎn)于新西蘭、澳大利亞.是從白干層屬植物中選用于林