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《脊髓刺激術(shù)對(duì)神經(jīng)病理性痛模型大鼠脊髓背角內(nèi)NR2B受體和星形膠質(zhì)細(xì)胞激活的影響.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在應(yīng)用文檔-天天文庫(kù)。
1、2013，29(3)：233—240神經(jīng)解剖學(xué)雜志ChineseJournalofNeuroanatomy脊髓刺激術(shù)對(duì)神經(jīng)病理性痛模型大鼠脊髓背角內(nèi)NR2B受體和星形膠質(zhì)細(xì)胞激活的影響張春奎，方相春，李志紅，唐君，董玉琳，李金蓮(第四軍醫(yī)大學(xué)人體解剖與組織胚胎學(xué)教研室暨梁鯨琚腦研究中心，西安710032)【摘要】目的：探討脊髓刺激術(shù)(spinalcordstimulation，SCS)對(duì)IJ5脊神經(jīng)結(jié)扎(spinalnerveligation，SNL)誘導(dǎo)的神經(jīng)病理性痛(neuropathicpain，NP)大鼠脊髓背角內(nèi)NMDA受體亞單位NR2B的表達(dá)和星形膠質(zhì)細(xì)胞激活的影響。方法
2、：成年雄性SD大鼠48只，隨機(jī)分為4組：正常組(不做任何處理)；SCS組(植入SCS裝置并給予SCS刺激)；SNL+shamSCS組(給予SNL手術(shù)并植入SCS裝置，但不進(jìn)行刺激)；SNL+SCS組(SNL手術(shù)并給予SCS刺激)。SCS刺激是在SNL術(shù)后第6—10d進(jìn)行(8h／d)，第10d刺激結(jié)束后處死動(dòng)物。運(yùn)用行為學(xué)方法檢測(cè)慢性痛狀態(tài)下大鼠后肢對(duì)機(jī)械性刺激的反應(yīng)閾值；采用免疫組織化學(xué)染色和Westernblot方法分別檢測(cè)脊髓背角內(nèi)NR2B和星形膠質(zhì)細(xì)胞的標(biāo)志物GFAP的表達(dá)變化。結(jié)果：(1)SNL術(shù)后大鼠手術(shù)側(cè)后足機(jī)械性痛敏顯著增加，第6～10d給予SCS刺激后，可觀察到大鼠的
3、痛行為學(xué)表現(xiàn)有明顯緩解；(2)免疫組化結(jié)果顯示：與SNL+shamSCS組相比，SNL+SCS組大鼠脊髓背角內(nèi)NR2B和GFAP免疫陽(yáng)性細(xì)胞的數(shù)量顯著減少；(3)Westernblot結(jié)果顯示：給予SCS刺激后，SNL大鼠腰膨大段脊髓背角內(nèi)NR2B的表達(dá)量顯著下調(diào)，同時(shí)GFAP的表達(dá)量也明顯有所降低。結(jié)論：給予SCS刺激可以有效地緩解SNL模型大鼠的神經(jīng)病理性痛的行為學(xué)表現(xiàn)；該作用可能與SCS刺激抑制脊髓背角內(nèi)NR2B的表達(dá)和星形膠質(zhì)細(xì)胞的激活密切相關(guān)?！娟P(guān)鍵詞】脊髓刺激術(shù)；神經(jīng)病理性痛；NR2B；星形膠質(zhì)細(xì)胞；大鼠TheeffectofSCSonNR2Breceptorandthe
4、activationofastrocytesinthespinaldorsalhornofratswithneuropathicpainZhangChunkui，F(xiàn)angXiangchun，LiZhihong，Jun，DongYulin，LiJinlian(DepartmentofAnatomy，HistologyandEmbryologyandK．K，LeungBrainResearchCentre，TheFourthMilitaryMedicalUniversity，Xi’an，710032)【Abstract】0bjective：Toinvestigatetheeffectof
5、spinalcordstimulation(SCS)onNR2BsubunitoftheNMDArecep—torandtheactivationofastrocytesinthespinaldorsalhornofratswithI5spinalnerveligationinducedneuropathicpain(NP)．Methods：48adultmaleratsweredividedinto4groupsrandomly：normalgroup(normalratswithoutanyopera—tion)；SCSgroup(normalratsplantedwithSCS
6、devices，andreceivedSCSstimulation)，SNL+shamSCSgroup(ratswereoperatedwithSNLandwereplantedwithSCSdevices，butdidnotreceiveSCSstimulation)，SNL+SCSgroup(SNLratswithSCS)．Duringthe6thto10thdayafterSNLoperation，ratsinSCSandSNL+SCSgroupsreceivedSCS，8honeveryday．AlltheratsweresacrificedafterSCSonthe10th
7、day．Behavioralmethodwasusedtodetectthehindpawwithdrawthresholdtomechanicalstimulationforratsinneuropathicpain．Then，theexpressionchangeofNR2BandGFAP(themarkproteinofastrocyte)inthespinaldorsalhornwasobservedviaimmunohisochemistryan
