大黃素對(duì)模擬冷缺血再灌注后肝細(xì)胞內(nèi)鈣離子濃度及細(xì)胞凋亡的影響

《大黃素對(duì)模擬冷缺血再灌注后肝細(xì)胞內(nèi)鈣離子濃度及細(xì)胞凋亡的影響》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。

1、大黃素對(duì)模擬冷缺血再灌注后肝細(xì)胞內(nèi)鈣離子濃度及細(xì)胞凋亡的影響作者：祁翔，呂毅，沈乃營(yíng)，劉昌，劉學(xué)民，王博祁翔【摘要】目的研究中藥大黃素對(duì)模擬冷缺血再灌注后肝細(xì)胞內(nèi)鈣離子濃度及細(xì)胞凋亡的影響。方法體外培養(yǎng)肝細(xì)胞株HL7702，隨機(jī)分為對(duì)照組和大黃素處理組。對(duì)照組未予大黃素處理，大黃素處理組按100、10、1μmol/L分為高、中、低3個(gè)濃度組，建立模擬冷缺血再灌注模型，流式細(xì)胞技術(shù)檢測(cè)各組細(xì)胞內(nèi)鈣離子濃度及細(xì)胞凋亡水平，分別檢測(cè)各組細(xì)胞培養(yǎng)上清液乳酸脫氫酶水平。結(jié)果冷缺血8h再灌注6h后，高、中、低濃度大黃素處理組鈣離子熒光強(qiáng)度分別為(24.12±0.51)、(26.35±1

2、.34)、(39.12±1.94)，均顯著低于對(duì)照組的105.29±1.01(P<0.01)。高、中、低濃度大黃素處理組細(xì)胞凋亡率分別為(5.46±0.41)%、(10.64±0.64)%、(11.90±0.50)%，均顯著低于對(duì)照組的(25.40±1.41)%(P<0.01)。高、中濃度大黃素處理組上清液LDH含量分別為(179.67±18.57)u/L、(198.83±14.22)u/L，顯著低于對(duì)照組的(351.33±34.16)u/L(P<0.01)。結(jié)論大黃素可降低模擬冷缺血再灌注后的肝細(xì)胞內(nèi)鈣離子濃度，抑制細(xì)胞凋亡，減輕肝細(xì)胞損傷?！娟P(guān)鍵詞】大黃

3、素；肝細(xì)胞；冷缺血再灌注；細(xì)胞內(nèi)鈣離子濃度；凋亡11　　ABSTRACT:ObjectiveToinvestigatetheeffectofEmodinonintracellularcalciumconcentration([Ca2+]i)andapoptosisofhepaticcellsaftersimulatedcoldischemiareperfusion.MethodsGlucoseoxygendeprivation,lowtemperature,subsequentreoxygenationandrewarmingwereusedtoinduceischemi

4、areperfusioninjurymodelinculturedhepaticcellswhichweredividedinto4groups:controlgroupandEmodintreatedgroup(100,10and1μmol/Lgroups).Theintracellularcalciumconcentration([Ca2+]i)andapoptosisrateweredeterminedbyflowcytometry(FCM)respectively;thecontentoflactatedehydrogenase(LDH)insupernatant

5、wastested.ResultsIntracellularcalciumfluorescenceintensityinEmodintreatedgroupsofhigh,mediumandlowdensitywas24.12±0.51,26.35±1.34and39.12±1.94,respectively,whichweresignificantlylowerthan105.29±1.01incontrolgroup(P<0.01).ApoptosisrateinEmodintreatedgroupsofhigh,mediumandlowdensitywas0.

6、0546±0.0041,0.1064±0.0064and0.1190±0.0050,respectively,whichweresignificantlylowerthan0.2540±0.0141incontrolgroup(P<0.01).LDHwas(198.83±14.22)u/Land(179.67±18.57)u/LinEmodintreatedgroupsofmediumandhighdensityrespectively,whichwere11significantlylowerthan(351.33±34.16)u/Lincontrolgroup(P

7、<0.01).ConclusionEmodincouldreduce[Ca2+]iandinhibitapoptosisofhepaticcellsaftersimulatedcoldischemiareperfusion,thusprotectinghepaticcellseffectively.　　KEYWORDS:Emodin;hepaticcell;ischemiareperfusioninjury;intracellularfreecalciumconcentration;apopt