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1、PreventiveVeterinaryMedicine,17(1993)263-269263ElsevierSciencePublishersB.V.,AmsterdamSeroprevalenceofporcinerespiratorycoronavirusinfectioninSpanishbreedingsowsIgnacioLanza*,PedroRubio,MdximoFermindez,MariaMufioz,PedroCdrmenesDepartamentodeSanidadAnimal(Enfermedadesinfecciosasy
2、Epidemiologia),FacultaddeVeterinaria,UniversidaddeLe6n,CampusdeVegazana,E-24071-Le6n,Spain(Accepted6April1993)AbstractTheseroprevalenceofporcinerespiratorycoronavirus(PRCV)amongbreedingsowswasesti-matedinCastillayLe6n,thelargestregioninSpain,byacross-sectionalstudy.Serumsamples(
3、1247)weretakenfromsowsfrom58differentherdsforthispurposethroughout1988andweretestedbyamonoclonalantibody-captureELISA(MACELISA),atestwhichdetectsantibodiestobothtrans-missiblegastroenteritisvirus(TGEV)andPRCV.Inordertodiscriminatepositiveseratobothco-ronaviruses,ablockinginhibit
4、ionELISAwasusedforallMACELISA-positivesera.ByMACELISA,31.4%ofseratestedwerepositiveandallofthemwereconfirmedspecificallyasPRCV-positivebyblockinginhibitionELISA,thusrulingoutthepresenceofTGEVinthesampledarea;64%ofthefarmshadatleastonePRCV-seropositivesow,indicatingthattheinfecti
5、onwaswidespreadFarmswereclassifiedintothreecategories,accordingtosizeandmanagementpractices.Nosignificantdif-ferenceswerefoundintheprevalenceratesamongthethreefarmtypes;therewas,however,ahighlysignificantcorrelationbetweenincreasingfarmsizeandincreasingwithin-farmseropositivity.
6、IntroductionPorcinerespiratorycoronavirus(PRCV),arespiratoryvariantoftrans-missiblegastroenteritisvirus(TGEV),wasfirstidentifiedinBelgiumin1986(Pensaertetal.,1986).PRCVismorphologicallyandantigenicallysimilartoTGEV,butithasnotropismforthegut,replicatingtohightitersintheres-pirat
7、orytissuesofthepigandnotinducingintestinaldisorders.Instead,itproducesmildorinapparentrespiratorysymptoms,anditaffectsthegrowthoffatteningpigs(Bourgueiletal.,1992;Lanzaetal.,1992).Thehumoralimmuneresponseelicitedbybothcoronavirusesisindistinguishable(duetocommonneutralizationepi
8、topes)bytheclassicalvirus-neutralizationtest,bu
