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1、醫(yī)護職稱的名與實評定職稱，有一個名與實的問題。關于名實的涵袁媛，司維柯，李招權，潘靜，趙宸【摘要】本研究探討外源性Wnt5a誘導K562細胞定向分化的作用。用重組腺病毒AdWnt5a、AdGFP感染CHO細胞，分別制備Wnt5a和GFP對照條件培養(yǎng)液，并處理K562細胞H7天，用光學顯微鏡和電子顯微鏡觀察細胞形態(tài)變化；采用過氧化物酶、糖原和非特異性酯酶染色、細胞表面分化抗原CD13、CD14、CD68鑒定K562細胞的分化表型；用流式細胞術檢測細胞分化周期的變化。結果表明：與對照GFP條件培養(yǎng)液相比，Wnt5a條件培養(yǎng)液處理的K562細胞形態(tài)和超微結構均出現(xiàn)了分化成熟的特征；POX、PAS
2、、aNAE細胞化學染色均為陽性，并且aNAE陽性70°/?？杀环c抑制；單核細胞系表面標志抗原CD14、CD68和粒細胞系表面標志抗原CD13經(jīng)免疫細胞化學染色均呈陽性，但CD13與對照比較無顯著性差異；Wnt5a處理K562細胞5天，細胞周期阻滯在G2期。結論：外源性的Wnt5a可誘導K562細胞向成熟方向分化，并且有向單核細胞系分化的現(xiàn)象?！娟P鍵詞】誘導分化DifferentiationPhenotypesofK562CellsInducedbyExogenousWnt5aAbstractThisstudywasaimedtoinvestigatetheeffectofexogeno
3、usWifferentiationofK5PconditionmediumsombinantadenoviralGFPtransfectingCHretreatedwithWnt5aonmediumsfori-7daoupandcontrolgrouporphologicalchangeservedbylightmicrocroscope；thediffersofK562cellswereidhemicalstainingofPOX，unocytochemistryofCD13，CD14，CD68，andncellcycledistribusdetectedbyflowcythowedth
4、atthemorphoureofK562cellstreeddifferentiationmPOXandPASstainingsveratioinWnt5atreant5aondirectionald62cells.Wnt5aandGFwerepreparedbyrecvectorAdWnt5aandAdOcells.K562cellsweandtheGFPconditiysasWnt5atreatedgrrespectively.ThemsofK562cellswereobscopeandelectronmientiationphenotypeentifiedbythecytocPAS，
5、aNAEandimmtheeffectofWnt5aotionofK562cellswaometry.TheresultsslogyandultrastructatedbyWnt5adisplayaturefeature；bothhowedhigherposititedgroupthanthatincontrolgroup；theaNAEstainingalsowaspositive,butpositiveintensityinWnt5atreatedgroupcouldbeinhibitedupto70%byNaF.Theexpressionsofmonocyticdifferentia
6、tionantigensofCD14,CD68inWnt5atreatedgroupwerehigherthanthoseincontrolgroup，buttheexpressiondifferencesofgranulocytiedifferentiationantigenCDl3betweenWnt5atreatedgroupandcontrolgroupwerenotsignificant.ThecellcycleintreatedgroupwasblockedatG2phaseascomparedwithcontrolgroup.Itisconcludedthatexogenou
7、sWnt5acaninduceK562cel1stodifferentiatetowardsmatureandK562cellstreatedwithWnt5adisplaysfeaturesofdifferentiationtowardsmonocyticlineage.KeywordsWnt5a；K562；inductiondifferentiation；WntsignalingJExpHematolXX；15(5)
