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1、不同基原秦皮、香豆素單體抗菌作用對比研究論文.freelarinmonomer,5kindsofmonomermixture,knoarinfingerprintregionsampleandunknoentinvitroandmicroamountandquantitativedetectionmethodseasureminimuminhibitoryconcentration(MIC)andminimumbactericidalconcentration(MBC).ResultFourkin
2、dsoforiginsashbarkincludingFraxinusrhynchophyllaHance,FraxinuschinensisRoxb.var.acuminataLingelsh.,Fraxinuschinensis,FraxinusstylosaLingelsh.hadobviousbacteriostasisandbactericidalaction,bacteriostasisintensityorderinataLingelsh.＞Fraxinuschinensis.Fiv
3、ekindsofcoumarinmonomer：fraxetin,aesculetinhadobviousbacteriostasisandbactericidalaction,aesculinandfraxinhadcertainbacteriostasisandbactericidalactiontoStaphylococcusaureus,Staphylococcusepidermidis.Bacteriostasisandbactericidalactionorderethoxyl-8-H
4、ydroxycoumarin.FraxinusrhynchophyllaHanceextracthadobviousbacteriostasisto9kindsofbacterium；monomermixtureandcoumarinfingerprintregionsamplehadobviousbacteriostasistoStaphylococcusaureus,Staphylococcusepidermidis,betastreptococcus,aeruginosusBacillus,
5、Bacilluscoli,Klebsiellapneumoniae,catarrh.UnknoplehadobviousbacteriostasistoStaphylococcusaureusandcertainbacteriostasistoStaphylococcusepidermidis,alphastreptococcus,betastreptococcus,Bacilluscoli,salmonella.ConclusionThechangesofashbarkbacteriostasi
6、sarinponentcontent.Aglyconebacteriostasisofashbarkostpoarinmonomer.Keyarinmonomer；bacteriostasis秦皮為木犀科植物苦櫪白蠟樹FraxinusrhynchophyllaHance、白蠟樹FraxinuschinensisRoxb.、尖葉白蠟樹FraxinusszaboanaLingelsh.、宿柱白蠟樹FraxinusstylosaLingelsh.的干燥枝皮或干皮,具有清熱燥濕、收澀、明目的功能,用于熱痢
7、、泄瀉、赤白帶下、目赤腫痛、目生翳膜等。秦皮主要含有香豆素類化學(xué)成分,如：秦皮甲素(aesculin)、秦皮乙素(aesculetion)、秦皮苷(fraxoside)、秦皮素(fraxetol)、6,7-二甲氧基-8-羥基香豆素1-2。本研究對4種基原秦皮提取物、5種香豆素單體、5種單體混合物和以苦櫪白蠟樹樹皮為代表的秦皮提取物、已知香豆素指紋區(qū)樣品、未知成分指紋區(qū)樣品進行了體外抑菌試驗,旨在闡明其抑菌、殺菌作用的強弱,為客觀地評價秦皮質(zhì)量、指導(dǎo)臨床用藥提供科學(xué)依據(jù)。1實驗材料1.1藥物4種基原
8、秦皮：苦櫪白蠟樹、尖葉白蠟樹、白蠟樹、宿柱白蠟樹樹皮提取物；5種香豆素單體：秦皮甲素、秦皮乙素、秦皮苷、秦皮素、6,7-二甲基-8-羥基香豆素(均為自制,純度＞98%)；以苦櫪白蠟樹為代表的秦皮提取物,5種香豆素單體混合物(按苦櫪白蠟樹提取物中各單體含量：秦皮甲素2.08%、秦皮乙素0.14%、秦皮苷0.84%、秦皮素0.37%、6,7-二甲基-8-羥基香豆素0.03%混合而成)；苦櫪白蠟樹已知香豆素指紋區(qū)樣品,未知成分指紋區(qū)樣品。上述樣品以DMSO溶解,實驗前配制。1.2試劑及培
