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1、摘要摘要本試驗(yàn)收集了生產(chǎn)上栽培的5個(gè)白靈菇菌株，通過拮抗試驗(yàn)、酯酶同工酶分析及出菇試驗(yàn)，對(duì)5個(gè)菌株進(jìn)行了生理生化特性和栽培特性分析。在親緣關(guān)系確定的基礎(chǔ)上，選取生產(chǎn)性狀優(yōu)劣互補(bǔ)的“中農(nóng)1號(hào)”和“中農(nóng)翅鮑”兩個(gè)菌株作為親本。出菇收集擔(dān)孢子，采用單孢分離技術(shù)分離兩個(gè)菌株的擔(dān)孢子，得到“中農(nóng)1號(hào)”單核體54個(gè)，“中農(nóng)翅鮑”單核體41個(gè)。選取生長較快的兩親本單核菌絲進(jìn)行了雜交配對(duì)，得到150個(gè)雜交組合菌株，根據(jù)鎖狀聯(lián)合有無進(jìn)一步確定融合子。采用拮抗、酯酶同工酶、原基誘導(dǎo)及出菇等方法對(duì)融合子進(jìn)行了進(jìn)一步鑒定和評(píng)價(jià)分析，結(jié)果表明：（1）通過拮抗、酯酶同工酶和出菇驗(yàn)證，5個(gè)白靈菇分
2、為兩大類群，一類以“中農(nóng)1號(hào)”為代表菌株；一類以“中農(nóng)翅鮑”為代表菌株。（2）通過鏡檢、拮抗、酯酶同工酶、原基誘導(dǎo)及出菇驗(yàn)證，150對(duì)雜交組合菌株中，99個(gè)菌株生長較快，有鎖狀聯(lián)合，75個(gè)與兩親本有明顯拮抗線，且具有兩親本菌株的典型酯酶同工酶條帶，確定是雜交菌株，其中50個(gè)雜交子具有出菇能力，出菇驗(yàn)證初步得到了3個(gè)優(yōu)良融合菌株。關(guān)鍵詞：白靈菇，單孢分離，雜交育種，篩選I萬方數(shù)據(jù)AbstractAbstractThephysiological,biochemicalandculturalcharacteristicsof5Pleurotuseryngiivar.tuol
3、iensisstrainswereanalysedbyantagonistictest,esteraseisozymeanalysisandfruitingtest.Using“zhongnong1”and“zhongnongchibao”withcomplementaryadvantageswereascrossingparentsonthefoundationofconfirminggeneticrelations.54monokaryonsfrom“zhongnong1”and41monokaryonsfrom“zhongnongchibao”wereobtai
4、nedbysinglesporeseparationtechnology.Twoabnormalsinglesporeisolateswerehybridizedandgot150hybridstrainaccordingtheexistenceofclamp-connection.Thefusantswereidentifiedbyantagonism,esteraseisozyme,primordiuminduction,fruitingtestandothermethods,theresultsshowedthat:(1)5Pleurotuseryngiivar
5、.tuoliensisweredividedintotwogreatcategoriesbyantagonism,esteraseisozymeandproducingmushroomdemonstration,onewastaken“zhongnong1”astherepresentativestrain;theotherwastaken“zhongnongchibao”astherepresentativestrain.(2)Among150hybridcombinationstrains,99strainsgrowedfasterwerewithclam-con
6、nection,75strainshadobviousantagonisticstreakswithtwoparents.equipwiththetypicalesyeraseisozymebeltoftwoparentstrainsbymicroscopicexamination,antagonism,esteraseisozymeandfruitingtestdemonstration.Theywereconfirmedashybridstrains,ofwhich50hybridscouldfruit.Thefruitingtestshowedthat3stra
7、inswereexcellentfusionhybrid.KeyWords:Pleurotuseryngiivar.tuoliensis,singlesporeseparation,hybridbreeding,screeningII萬方數(shù)據(jù)目錄目錄摘要..............................................................IAbstract..........................................................Ⅱ第一章緒論................
