資源描述:
《超聲波法虎杖中總黃酮提取工藝研究》由會員上傳分享,免費在線閱讀,更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、寶雞文理學(xué)院學(xué)報(自然科學(xué)版),第33卷,第4期,第31—35,39頁,2013年12月JournalofBaojiUniversityofArtsandSciences(NaturalScience),Vo1.33,No.4,PP.31—35,39,Dec.2013D0I:61-1290/N.20131105.1116.001http:f?ww.cnki.net/kcms/detail/61.1290.N.20131105.116.001.html超聲波法虎杖中總黃酮提取工藝研究胡春霖,鄭可利,羅利琴,陳惠玲,雷伙華,陸該妹(三明學(xué)
2、院資源與化工學(xué)院,福建三明365004)摘要:目的確定超聲波輔助方法提取虎杖中總黃酮的最佳工藝。方法采用正交實驗優(yōu)化影響虎杖中總黃酮類成分提取的4種主要因素:提取劑(CH。CHOH)濃度、提取溫度、超聲時間以及料液比;并以蘆丁為標(biāo)準(zhǔn)樣品,利用紫外可見分光光度法測定虎杖中總黃酮的吸光度值,為確定最佳提取條件提供參考。結(jié)果通過正交實驗得到從虎杖中提取總黃酮的最佳工藝為:乙醇濃度為75%,超聲溫度為5O℃,超聲時間為35min,料液比1:40,其提取率可以達(dá)到7.68。結(jié)論通過該實驗確立了虎杖中總黃酮提取的最優(yōu)工藝,提取效果良好、操作簡便易
3、行,節(jié)能環(huán)保。關(guān)鍵詞:虎杖;總黃酮;超聲提取;正交實驗中圖分類號:R284.2文獻標(biāo)志碼:A文章編號:1007—1261(2013)04—003卜05ExtractionprocessresearchofflavonoidsfromPolygonumcuspidatumwithultrasonicmethodHUChun-lin,ZHENGKe-li,LUOLi-qin,CHENHui—ling,LEIHuo-hua,LUGai-mei(CollegeofResourcesandChemicalEngineering,SanmingU
4、niversity,Sanming365004,F(xiàn)ujian,China)Abstract:Objective-TodeterminetheoptimumprocessofextractingtotalflavonoidsfromPolygonumcuspidatumbyusingtheultrasonicextractiontechnology.Methods-TheorthogonaldesignexperimentisusedtOoptimizeandinfluencethefourdominantfactors(thecont
5、entofextrac—tionagent(CH3CH2OH),extractiontemperature,ultrasonictimeandsolid—liquidratio)ofextractingtotalflavonoids.Thenrutinwasasareferenceobject,theultraviolet—visiblespectrophotometryisuti—lizedtodeterminetheabsorbancevalueoftotalflavonoidsinPolygonumcuspidatumtopro
6、videthereferenceforoptimalextractionparameters.Results—TheresearchresultsshowthattheextractionefficiencyoftotalflavonoidsiS7.68%,whentheethanolconcentrationiS75,theultrasonictimeiS35min,thetemperatureis50℃andthesolid—liquidratioisonetoforty.Conclusion-Throughtheex—perim
7、ent,notonlyaretheoptimalextractionconditionsestablishedbutalsotheextractioneffectisterrific.Moreover,theexperimentissimpleandeasyaswellasenergy-savingandenvironment-friendly.Keywords:Polygonumcuspidatum;totalflavonoids;ultrasonicextraction;orthogonaldesignexperiment虎杖(P
8、olygonumcuspidatumSieb.etZucc)陰暗潮濕的地方如山溝、溪邊、山坡及林下等,主為蓼科蓼屬多年生灌木狀草本植物,又名斑杖、斑要分布于我國福建、湖北、陜西及四川I等地?;⒄雀白辖瘕垺V饕L在海拔