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1、tm040.0806.qxp8/11/20062:49PMPage1TechnicalManualDual-Luciferase?ReporterAssaySystemINSTRUCTIONSFORUSEOFPRODUCTSE1910ANDE1960.PRINTEDINUSA.Revised8/06Part#TM040AF9TM0400806TM040tm040.0806.qxp8/11/20062:49PMPage1Dual-Luciferase?ReporterAssaySystemAlltechnicallite
2、ratureisavailableontheInternetat:www.promega.com/tbs/PleasevisitthewebsitetoverifythatyouareusingthemostcurrentversionofthisTechnicalManual.PleasecontactPromegaTechnicalServicesifyouhavequestionsonuseofthissystem.E-mail:techserv@promega.com.I.Description........
3、..................................................................................................2A.Dual-Luciferase?ReporterAssayChemistry...................................................3B.FormatoftheDual-Luciferase?ReporterAssay.............................
4、................5C.PassiveLysisBuffer.............................................................................................6II.ProductComponentsandStorageConditions............................................8III.ThepGL4LuciferaseReporterVectors..........
5、...............................................9A.DescriptionofpGL4Vectors..............................................................................9B.ImportantConsiderationsforCo-TransfectionExperiments........................9IV.InstrumentConsiderations...
6、.........................................................................10A.Single-SampleLuminometers...........................................................................10B.Multi-SampleandPlate-ReadingLuminometers.........................................
7、.10C.ScintillationCounters.........................................................................................11V.PreparationofCellLysatesUsingPassiveLysisBuffer..........................12A.PassiveLysisBufferPreparation.....................................
8、................................12B.PassiveLysisofCellsCulturedinMultiwellPlates.....................................12C.ActiveLysisofCellsbyScraping.................
